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INSTRUMENTATION [1]

Paper Code: 
BTE 124
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

 

          Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 17: Acquire knowledge of Bioinstrumentation

CO 18: Understand the laws of Spectroscopy and basic principles of Spectroscopic techniques.

CO 19: Employ analytical reseasoning in biological sample separation through Centrifugation techniques

CO 20: Apply scientific knowledge to address research problems using electrophoretic separation techniques.

CO 21: Employ scientific reasoning in analyzing and addressing practical problemsin Chromatography

CO 22: Acquire the ability to engage in research and development using Microscopic analysis

 

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

 
 

 

15.00
Unit I: 
Spectroscopy
  • Beer-Lambert law, UV-Vis spectroscopy, fluorescence spectroscopy, IR spectroscopy, Raman spectroscopy, Atomic absorption spectroscopy, Mass spectroscopy, NMR, ESR, CD/ORD, MALDI-ToF-MS, X-ray diffraction.

 

 

15.00
Unit II: 
Chromatography
  • Principle, Paper chromatography, TLC, Column chromatography (GC, HPLC), Adsorption chromatography, Partition chromatography, Gel filtration, Ion-exchange chromatography and Affinity chromatography.
  • Special note on GCMS/LCMS

 

15.00
Unit III: 
Electrophoresis
  • Principle, factors affecting electrophoresis, pH, voltage, supporting medium (agarose, polyacrylamide, dextran).
  • Agarose gel electrophoresis, PAGE, SDS-PAGE, 2-D electrophoresis, Pulsed field gel electrophoresis, isoelectric focusing, immuno electrophoresis.

 

15.00
Unit IV: 
Centrifugation & Radioisotopes
  • Centrifugation: principle differential and density gradient, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF.
  • Radioisotopes; nature of radioactivity, types of radioactive decay, unit of radioactivity.
  • Autoradiography, Cerenckov radiation, liquid scintillation counting, Geiger-Muller counter.

 

15.00
Unit V: 
Microscopy
  • Microscopy: construction of a microscope, light microscopy (bright field, dark field, phase-contrast, interference, confocal, polarization microscopy).
  • Electron microscopy-TEM, SEM, Scanned probe microscopic techniques (STEM, AFM)
  • Cytophotometry, flow cytometry, Cryo-electron microscopy.

                                                                  

 

ESSENTIAL READINGS: 

ESSENTIAL READINGS:

  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2007.
  • Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc., 2009
  • Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2008
  • Principles and Techniques of Biochemistry and  Molecular Biology ,(7th edition), K Wilson and J Walker (editor), Cambridge University Press, 2010

SUGGESTED READINGS:

  • Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2014
  • Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005  

 

REFERENCES: 

e RESOURCES:

JOURNALS:

  • Indian Journal of Experimental Biology
  • Journal of Scientific and Industrial Research

 

 

 

 

Academic Year: 
2022-2023 [5]

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Source URL: https://biotech.iisuniv.ac.in/courses/subjects/instrumentation-13

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/instrumentation-13
[2] http://nopr.niscair.res.in/
[3] https://ndl.iitkgp.ac.in/homestudy/cbse_exam/x_subject/mathematics#page-2
[4] http://ndl.iitkgp.ac.in/document/Z2JzN0ZmU2VhdW5kODBJdWRCTmg3TWVkYWdIL0NoMEMzaEo2Z0V1dU5WSWF1d3RMNHhiRHZVSlJKVzRVSFdGWkpXck9HUHpaWHJQRzF4a2pKVFlNRG12V3dlRjlGd0RnYld5MVZLWUdGVkE9
[5] https://biotech.iisuniv.ac.in/academic-year/2022-2023