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GENETIC ENGINEERING [1]

Paper Code: 
BTE 224
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course the learner will:

CO 59: Learn the basics of Genetic Engineering and study different cloning and expression vectors which will help to build their concrete concepts about the subject.

CO 60: Apply enzymes used in Genetic Engineering in the procedures involved in isolation of genomic and nuclear DNA.

CO 61: Use tools and techniques of the PCR, hybridization, DNA sequencing procedures for creating new and improving existing issues in the field of medicine.

CO 62: Apply tools and techniques for the production of therapeutic proteins; solve cases by genetic counselling and vaccine development.

CO 63: Apply their knowledge for improving and developing various crop varieties in the field of agriculture.

CO 64: Solve issues related to Forensic science, improvement of existing industrial processes and contribute to society as a whole.

Interactive Lectures, Discussion, Tutorials, Reading assignments, Self-learning assignments, Effective questions, Giving tasks

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  
 

 

17.00
Unit I: 
Tools of genetic engineering
  • Scope and milestones in genetic engineering, Basic tools and techniques used in recombinant DNA technology: Restriction endonuclease, DNA modifying enzymes, cloning vectors: plasmids, bacteriophage, cosmid, phagemids, YAC, BAC, in vitro construction of vectors, expression vectors.
  • Linkers and Adaptors. Principle and uses of nucleic acid hybridization. Principle and applications of polymerase chain reaction, Q-PCR.                                                                                                                        

 

15.00
Unit II: 
Sequencing of genes
  • DNA and genomic library: m- RNA enrichment, construction and screening of c DNA and genomic library, Genome sequencing:  strategies of genome sequencing, Sequencing vector, fluorescent tagging, Pyrosequencing, Automated DNA sequencing, Introduction to Next generation sequencing technologies.

 

13.00
Unit III: 
Molecular Mapping of Genome
  • Genetic and physical mapping, Restriction mapping and map construction, Analysis of genetic variations: RAPD, RFLP, AFLP and other molecular marker techniques, application of RFLP in forensic studies, disease prognosis, genetic counselling, pedigreeanalysis.                                                                                                                     

 

 

13.00
Unit IV: 
Gene delivery Techniques, Gene Therapy and Patenting
  • Direct gene delivery methods and Agrobacterium mediated transformation techniques, Gene therapy: Target gene replacement, gene knockout technique, Genome editing techniques (ZFN, TALEN, CRISPR/Cas9) computer aided drug designing. Patenting of cloned life forms
17.00
Unit V: 
Gene expression and Analysis
  • Site directed mutagenesis and protein engineering. in vitro DNA synthesis, in vitro transcription and translation. Heterologous gene expression in bacteria, yeasts, insects, mammals and plants, codon optimization, DNA and protein microarray technology, RNase protection assay, Reporter gene assay, Western blotting, S1 nuclease assay.

 

ESSENTIAL READINGS: 

ESSENTIAL READINGS:

  • Brown, T. A., Gene Cloning and DNA Analysis, An Introduction.  Wiley –Blackwell publication. 2010.
  • Sandy B. Primrose, Richard M. Twyman.  Principles of Gene Manipulation and Genomics, Blackwell Sciectific Publication. 2009.
  • Brown, T. A., Genomes (3rd ed.). New York: Garland Science Pub. 2006.
  • Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.
  • Green, M. R., & Sambrook, J., Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 2012.

SUGGESTED READINGS:

  • P.B. Kaufman, W. Wu. D. Kim and L.J; Cseke, Molecular and Cellular Methods in Biology and Medicine, CRC Press, Florida.
  • M. Glover and B.D. Hames, DNA Cloning: a Practical Approach, IRL Press, Oxford.
  • S.L. Berger and A.R. Kimmel, Methods in Enzymology vol. 152, Guide to Molecular Cloning Techniques, Academic Press, Inc. San Diego.
  • D.V. Goeddel, Methods in Enzymology Vol 185, Gene Expression Technology, Academic Press, Inc., San Diego.
  • Kingsman S.M. and Kingsman A.J., Genetic Engineering. An Introduction to gene analysis and exploitation in eukaryotes. Blackwell Scientific Publications, Oxford, 1990.
  • Desmond S.T. Nicholl, An Introduction to Genetic Engineering. Edited by, Cambridge University Press. 2002.
  • Sue Carson and Dominique Robertson, Manipulation and Expression of Recombinant DNA., Second edition, Academic Press. 2005.
  • Primrose and Twyman, Principles of Gene Manipulation and Genomics. (7th edition). Blackwell Publishers. 2006.

 

REFERENCES: 

       e RESOURCES:

JOURNALS:

  • International Journal of Molecular Biotechnology
  • Indian Journal of Biotechnology
  • Current Science

 

Academic Year: 
2022-2023 [7]

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Source URL: https://biotech.iisuniv.ac.in/courses/subjects/genetic-engineering-18

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/genetic-engineering-18
[2] https://nptel.ac.in/courses/102103013
[3] https://nptel.ac.in/courses/102103074
[4] https://www.youtube.com/watch?v=3IsQ92KiBwM
[5] https://www.youtube.com/watch?v=FtNreXY7poA
[6] https://www.youtube.com/watch?v=FvHRio1yyhQ&t=71s
[7] https://biotech.iisuniv.ac.in/academic-year/2022-2023