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BACTERIOLOGY [1]

Paper Code: 
MBL 202
Credits: 
03
Contact Hours: 
45
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO 22: identify the role of microbes in different fields

CO 23: evaluate bacterial growth, calculate generation time and analyze the growth kinetics of bacterial culture

CO 24: evaluate the different types of methods for controlling microbial growth.

CO 25: design and formulate different types of medium for microbial growth

CO 26: differentiate, compare and use different types of staining methods and preservation.

Interactive Lectures,

Discussion,

Tutorials,

Demonstrations,

Assignments

Reading JOURNALS:

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

9.00
Unit I: 
Scope of Microbiology
  • Microbiology as a field of biology, different branches of microbiology, a brief idea of some applied areas of microbiology: medical microbiology, food, agriculture and industrial microbiology.

 

10.00
Unit II: 
Growth of bacteria
  • Definition of growth, rate of growth, generation time, growth curve, measurement of population growth, batch culture, continuous culture and synchronous growth.
  • Factors affecting growth. - Temperature, pH, oxygen, water availability.

 

9.00
Unit III: 
Control of Microorganisms
  • Physical agents- Moist heat, dry heat, filtration, radiation.
  • Chemicals as antimicrobial agents major groups of disinfectants, antiseptics and chemical sterilants.

 

8.00
Unit IV: 
Cultivation of microorganisms
  • Microbiological media, Physical states and chemical content of media, general purpose and special purpose media. Nutritional classification of Microorganisms.
  • Isolation techniques (Streak plate, pour plate and spread plate method).

 

9.00
Unit V: 
Identification and preservation of microorganisms
  • Preparation of microorganisms for light microscopy- wet mount and hanging drop technique. Staining techniques- simple staining, differential staining (Gram’s staining, acid fast staining, endospore staining). Special staining (capsule staining and flagella staining).
  • Preservation of microorganisms by sub culturing, overlaying cultures with mineral oil, lyophilization, use of liquid nitrogen.

 

ESSENTIAL READINGS: 
  • Prescott’s Microbiology, Joanne Willey, Linda Sherwood, Christopher J. Woolverton, 9th edition,McGraw-Hill Education, 2013.
  • Microbiology-a Laboratory Manual, 9th edition, J G Cappuccino and N C Sherman, Benjamin Cummings, 2010
  • Microbiology-an introduction, 12th edition G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2016
  • Microbiology ,5th edition M J Pelczar, E C S Chan, N R Kreig, New Delhi, Tata Mc Graw Hill, 2010
  • Microbiology: Principles and Explorations, Jacquelyn G. Black, Laura J. Black 9th edition, Wiley, 2015

 

REFERENCES: 

SUGGESTED READINGS:

  • Brock biology of microorganisms. Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A.16th ed Hoboken, NJ : Pearson Education, 2021
  • Microbiology A Human Perspective, Nester E.W., Roberts CE, Nester MT, Wm. C. McGraw-Hill Medical Publishing, 2009
  • Lippincott Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Richard A. Harvey, Cynthia Nau Cornelissen, Third, North American editionLWW, 2012
  • Alcamo's Laboratory Fundamentals Of MicrobiologyJeffrey C. Pommerville 9th edition, Jones and Barlett Publishers, 2010

 

e RESOURCES:

JOURNALS:

  • Journal of Bacteriology
  • Indian Journal of Microbiology
  • Journal of Applied Microbiology

 

Academic Year: 
2022-2023 [8]

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Source URL: https://biotech.iisuniv.ac.in/courses/subjects/bacteriology-13

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/bacteriology-13
[2] https://www.scientificbio.com/what-are-the-stages-of-the-bacterial-growth-curve/
[3] http://www.generalmicroscience.com/category/microbiology/
[4] https://milnepublishing.geneseo.edu/suny-microbiology-lab/chapter/differential-staining-techniques/
[5] https://www.ncbi.nlm.nih.gov/books/NBK562156/
[6] https://orbitbiotech.com/isolation-of-bacterial-cultures-pure-culture-bacterial-culture-serial-dilution-spread-plate-streak-plate/
[7] https://microbenotes.com/classification-of-bacteria-on-the-basis-of-nutrition/
[8] https://biotech.iisuniv.ac.in/academic-year/2022-2023