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LABORATORY II [1]

Paper Code: 
MBL 226
Credits: 
08
Contact Hours: 
240
Objective: 

Course Outcomes (COs):

          Learning outcome

          (at course level)

Learning and teaching strategies

Assessment Strategies

 

Upon completion of the course the learner will:

CO 68: Learn experiments related to Molecular Biology, Microbial Genetics and Genetic Engineering.

CO 69: Learn demonstrations and experiments related to Immunology

CO 70: Learn experiments related to Virology, Mycology and Phycology

Discussion, Tutorials

Effective questions, Simulation, Giving tasks, Practical

 

Continuous Assessment

Semester End Exam

CONTENTS:

  1. Isolation of antibiotic resistant microorganisms by replica plating.
  2. Isolation of antibiotic resistant microorganisms by Gradient plate technique.
  3. Effect of U.V. rays on bacteria.
  4. Isolation of plasmid DNA 
  5. Isolation of genomic DNA
  6. Spectrophotometric quantitation of nucleic acids
  7. Ouchterlony double diffusion
  8.  ELISA
  9. Demonstrations:
    1. Sandwich ELISA
    2. Latex agglutination
    3. Rocket Immunoelectrophoresis
  10. Restriction digestion of λDNA
  11. Ligation
  12. Transformation of DH5α (including preparation of competent cells)
  13. Demonstartions
  14. Conjugation
  15.   Reporter gene assay (GFP)/ Bacterial Transduction
  16. Study of various symptoms produced in plants due to virus infection.
  17. Study of viral diseases of plants/animals/human (Specimen/photographs)
  18. Different type of viruses (Photographs/sketches).
  19. Titration of Phages
  20. Isolation and identification of fungi.
  21. study of permanent slides of algae and fungi 
  22. Isolation and identification of Rhizobium from root nodules of leguminous plants.
  23. Isolation and identification of Azotobacter from soil.
  24. Experimental verification that absorption at OD260 is more for denatured DNA than native ds DNA

 

Academic Year: 
2022-2023 [2]

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Source URL: https://biotech.iisuniv.ac.in/courses/subjects/laboratory-ii-24

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/laboratory-ii-24
[2] https://biotech.iisuniv.ac.in/academic-year/2022-2023