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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO43A: Understand the principles of cell culture techniques Study different types of transport mechanisms. CO44A: Identify required conditions for cell cultures, knowing of media and the equipment required for cell culture. CO45A: understand the cell culture problems and possibilities. CO46A: Apply the knowledge of commercial aspect of Animal Biotechnology CO47A: Learn the application of cell hybridization. |
Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations Self-learning assignments, Effective questions, Seminar presentation, Giving tasks. |
C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction |
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Structure, organization and biology of animal cell
Cytoskeleton: Self-assembly and dynamic structure of cytoskeletal filaments, actin filament, microtubules
Cell –Cell interaction: cell adhesion, tight and gap junctions, plasmodesmata.
General concepts of animal cell culture: Proliferation, differentiation and dedifferentiation
Cell Culture laboratory: basic requirement, set up
Media -Development of simple growth media, BSS
Chemical, physical and metabolic functions of different constituents of culture medium
-Role of CO2 and serum
Serum and protein free defined media and their applications
Growth kinetics of cultured cells
Primary culture and cell line formation, Cell lines: A general concept, finite and infinite cell lines
Organ, organotypic and histotypic cultures
Cell separation
Cell cloning and micromanipulation
Cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC
Enzymes used in gene manipulation- DNA polymerase, Polynucleotide kinase, ligase, terminal deoxynucleotidyl transferase, Reverse transcriptase, Restriction endonucleases
Transfection techniques
Blotting Techniques- Southern, Northern and Western Blotting
Expression of proteins in animal cells: GISH, FISH, microarray
Scaling-up of animal cells
Cell culture-based vaccines
Stem cell culture, embryonic stem cells and their applications
Cell hybridization, Hybridomas and their selection, application of cell hybridization
3 D printing
• Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley-Liss. 2005.
• Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000
• Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998
• Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
• Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
• Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003.
Suggested Reading:
• Animal Cell Culture by Mohamed Al-Rubeai, Springerlink 2015.
• Animal Cell Culture: Essential Methods, Edited by Davis, Wiley 2011
e Resources:
· https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.2004.494032050381 [2]
· https://iopscience.iop.org/book/978-0-7503-1347-6/chapter/bk978-0-7503-1347-6ch1 [3]
· https://www.deshbandhucollege.ac.in/pdf/resources/1589512953_Z(H)-VI-Biotech- [4]4.pdf [4]
· https://www.sciencedirect.com/book/9780750604215/animal-cell-technology [5]
· https://nptel.ac.in/courses/102108086 [6]
• https://epgp.inflibnet.ac.in/Home [7]
JOURNALS:
• Animal Biotechnology, Elsevier Public Health Emergency Collection [8]
• Biochemistry and Molecular Biology Education, IUBMB JOURNALS:
• Advances in Bioscience and Biotechnology [9]
• Artificial Cells, Nano medicine and Biotechnology, Taylor and Francis [10]
Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/animal-biotechnology-and-recombinant-dna-technology
[2] https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.2004.494032050381
[3] https://iopscience.iop.org/book/978-0-7503-1347-6/chapter/bk978-0-7503-1347-6ch1
[4] https://www.deshbandhucollege.ac.in/pdf/resources/1589512953_Z(H)-VI-Biotech-4.pdf
[5] https://www.sciencedirect.com/book/9780750604215/animal-cell-technology
[6] https://nptel.ac.in/courses/102108086
[7] https://epgp.inflibnet.ac.in/Home
[8] https://www.ncbi.nlm.nih.gov/pmc/?term=Elsevier%20Public%20Health%20Emergency%20Collection%5bfilter%5d
[9] https://www.scirp.org/journal/journalarticles.aspx?journalid=164
[10] https://www.ncbi.nlm.nih.gov/pubmed/29265888
[11] https://biotech.iisuniv.ac.in/academic-year/2023-2024