UNITS

TOPICS

TEACHING HOURS

Unit I

RECOMBINANT DNA TECHNOLOGY -1

Principles and methods of recombinant DNA technology-hybridization, Cloning strategies:Cutting and joining DNA molecules, c DNA and genomic DNA libraries , screening strategies, cloning in E. coli, plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors.

12

Unit II

RECOMBINANT DNA TECHNOLOGY – II

Polymerase chain reaction (PCR), DNA sequencing, Molecular markers- Non PCR based: RFLP, PCR based: RAPD, AFLP, SSR or Microsatellites, DNA Fingerprinting Genome projects-Arabidopsis genome, Rice Genome.

12

Unit III

TRANSGENESIS

Concept and history of transgenesis in plants, principles and techniques of gene cloning: direct DNA transfer to plant cells, Agrobacterium mediated transformation: Ti plasmid, process of T-DNA transfer and integration, vectors, promoter, terminator, marker and reporter genes, ethical and ecological issues of transgenesis, terminator technology (GURT)

12

Unit IV

TRANSGENICS IN CROP IMPROVEMENT

Resistance to Biotic Stress: Insect Resistance, Virus resistance and Disease resistance; Resistance to Abiotic stresses; Herbicide resistance; Transgenics for Quality improvement: improved storage, longer life flowers, flower colour and shape and male sterility; commercial transgenic crops.

12

Unit V

INTELLECTUAL PROPERTY  RIGHTS

History and evolution of IPR, forms of IPR: patent, design and copyright; biotechnological Patent Facilitating Cell (BPFC), distinction    among various forms of IPR, rights/ protection, infringement or violation remedies against infringement (civil and criminal), Indian Patent Act.

12