Bacteriology

Published on Biotechnology (https://biotech.iisuniv.ac.in)

Bacteriology [1]

Paper Code:

MBL 202

Credits:

Contact Hours:

Objective:

This paper will enable students to understand –

The nature of biological material and hierarchy of molecular organization of cells.
The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

Course Outcomes (COs):

Course		Learning outcome (at course level)	Learning and teaching strategies	Assessment Strategies
Paper Code	Paper Title	Learning outcome (at course level)	Learning and teaching strategies	Assessment Strategies
		CO 24	Approach in	C A test,
		To have an idea about different	teaching:	Semester
		aspects of applied microbiology	Interactive	end
		CO 25	Lectures,	examinations
		To understand the	Discussion,	, Quiz,
		phenomena of bacterial	Tutorials,	Solving
		growth and factors	Reading	problems in
		affecting it.	assignments,	tutorials,
		CO 26	presentations	Assignments,
MBL 202	Bacteri ology	To learn staining techniques and preservation of microbes	Learning activities for	Classroom interaction
		CO 27	the students:
		To learn aseptic	Self-learning
		techniques	assignments,
		CO 28	Effective
		To learn the different	questions,
		types of microbiological	Seminar
		media	presentation,
			Giving tasks.

9.00

Unit I:

Scope of Microbiology

Microbiology as a field of biology, different branches of microbiology, a brief idea of some applied areas of microbiology: medical microbiology, food, agriculture and industrial microbiology.

10.00

Unit II:

Growth of bacteria

Definition of growth, rate of growth, generation time, growth curve, measurement of population growth, batch culture, continuous culture and synchronous growth.
Factors affecting growth. - Temperature, pH, oxygen, water availability.

9.00

Unit III:

Control of Microorganisms

Physical agents- Moist heat, dry heat, filtration, radiation.
Chemicals as antimicrobial agents major groups of disinfectants, antiseptics and chemical sterilants.

8.00

Unit IV:

Cultivation of microorganisms

Microbiological media, Physical states and chemical content of media, general purpose and special purpose media. Nutritional classification of Microorganisms.
Isolation techniques (Streak plate, pour plate and spread plate method).

9.00

Unit V:

Identification and preservation of microorganisms

Preparation of microorganisms for light microscopy- wet mount and hanging drop technique. Staining techniques- simple staining, differential staining (Gram’s staining, acid fast staining, endospore staining). Special staining (capsule staining and flagella staining).
Preservation of microorganisms by sub culturing, overlaying cultures with mineral oil, lyophilization, use of liquid nitrogen.

ESSENTIAL READINGS:

General Microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
Microbiology ,5th edition M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2006
Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006
Microbiology-an introduction, 9th edition G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2007
Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
Laboratory fundamentals of microbiology, I E Alcamo, Jones and Barlett Publishers, 2001
Microbiology , 6th edition, Prescott, Harley, Klein, Mc Graw Hill Companies, 2005
Microbiology Fundamentals and Applications ,2nd edition, R M Atlas, Maxwell Macmillan International Edition, 1989

Academic Year:

Source URL: https://biotech.iisuniv.ac.in/courses/subjects/bacteriology-10

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/bacteriology-10
[2] https://biotech.iisuniv.ac.in/academic-year/2021-2022