Bacteriology

Paper Code: 
MBL 202
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to understand –

 

  1. The nature of biological material and hierarchy of molecular organization of cells.
  2. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
  3. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

 

CO 24

Approach       in

C     A     test,

 

 

To have an idea about different

teaching:

Semester

 

 

aspects of applied microbiology

Interactive

end

 

 

CO 25

Lectures,

examinations

 

 

To       understand        the

Discussion,

,            Quiz,

 

 

phenomena of bacterial

Tutorials,

Solving

 

 

growth       and       factors

Reading

problems    in

 

 

affecting it.

assignments,

tutorials,

 

 

CO 26

presentations

Assignments,

 

MBL 202

Bacteri ology

To        learn        staining

techniques                  and preservation of microbes

 

Learning activities                 for

Classroom interaction

 

 

CO 27

the students:

 

 

 

To         learn         aseptic

Self-learning

 

 

 

techniques

assignments,

 

 

 

CO 28

Effective

 

 

 

To    learn    the    different

questions,

 

 

 

types of   microbiological

Seminar

 

 

 

media

presentation,

 

 

 

 

Giving tasks.

 

 

9.00
Unit I: 
Scope of Microbiology
  • Microbiology as a field of biology, different branches of microbiology, a brief idea of some applied areas of microbiology: medical microbiology, food, agriculture and industrial microbiology.

 

10.00
Unit II: 
Growth of bacteria
  • Definition of growth, rate of growth, generation time, growth curve, measurement of population growth, batch culture, continuous culture and synchronous growth.
  • Factors affecting growth. - Temperature, pH, oxygen, water availability.

 

9.00
Unit III: 
Control of Microorganisms
  • Physical agents- Moist heat, dry heat, filtration, radiation.
  • Chemicals as antimicrobial agents major groups of disinfectants, antiseptics and chemical sterilants.

 

8.00
Unit IV: 
Cultivation of microorganisms
  • Microbiological media, Physical states and chemical content of media, general purpose and special purpose media. Nutritional classification of Microorganisms.
  • Isolation techniques (Streak plate, pour plate and spread plate method).

 

 

9.00
Unit V: 
Identification and preservation of microorganisms
  • Preparation of microorganisms for light microscopy- wet mount and hanging drop technique. Staining techniques- simple staining, differential staining (Gram’s staining, acid fast staining, endospore staining). Special staining (capsule staining and flagella staining).
  • Preservation of microorganisms by sub culturing, overlaying cultures with mineral oil, lyophilization, use of liquid nitrogen.

 

ESSENTIAL READINGS: 
  • General Microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
  • Microbiology ,5th edition M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2006
  • Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006
  • Microbiology-an introduction, 9th edition G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2007
  • Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
  • General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Laboratory fundamentals of microbiology, I E Alcamo, Jones and Barlett Publishers, 2001
  • Microbiology , 6th edition, Prescott, Harley, Klein, Mc Graw Hill Companies, 2005
  • Microbiology Fundamentals and Applications ,2nd edition, R M Atlas, Maxwell Macmillan International Edition, 1989

 

Academic Year: 
2021-2022
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