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LABORATORY VA [1]

Paper Code: 
DBTE 502A
Credits: 
2
Contact Hours: 
60
Objective: 

               Course Outcomes

Learning and teaching strategies

Assessment Strategies

  
 

On completion of this course, the learner will:

CO48A: Acquire knowledge animal cell culture laboratory.

CO49A: Accomplish Cell viability assay Restriction Digestion of Lambda DNA, Ligation of Lambda DNA, plasmid isolation

CO50A: Analyze cell viability and enumeration of cells through hemocytometer.

CO51A:Acquire the knowledge of working of PCR

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  

 

1.    Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory

2.    Hemocytometry

3.    Cell viability assay

4.    Restriction Digestion of Lambda DNA

5.    Ligation of Lambda DNA

6.    Demonstration of PCR

7.    Plasmid isolation

Academic Year: 
2023-2024 [2]

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IIS(deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158

Source URL: https://biotech.iisuniv.ac.in/courses/subjects/laboratory-va-0

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/laboratory-va-0
[2] https://biotech.iisuniv.ac.in/academic-year/2023-2024