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LABORATORY VA
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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On completion of this course, the learner will: CO48A: Acquire knowledge animal cell culture laboratory. CO49A: Accomplish Cell viability assay Restriction Digestion of Lambda DNA, Ligation of Lambda DNA, plasmid isolation CO50A: Analyze cell viability and enumeration of cells through hemocytometer. CO51A:Acquire the knowledge of working of PCR |
Class lectures Seminars Tutorials Group discussions and Workshops Question preparation
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Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
1. Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory
2. Hemocytometry
3. Cell viability assay
4. Restriction Digestion of Lambda DNA
5. Ligation of Lambda DNA
6. Demonstration of PCR
7. Plasmid isolation
