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Laboratory VIII [1]

Paper Code: 
DBTE 704
Credits: 
2
Contact Hours: 
60
Objective: 

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 

 

Upon completion of the course the learner will:

CO77: Understand and relate to various genetic engineering techniques.

CO78:  Use and illustrate various gene transfer techniques like conjugation and transformation

CO79: Plan to prepare competent cells

CO80: Pivot after understanding the principle underlying Gel electrophoresis and SDS page

CO81: Appraise knowledge to isolate Plasmid DNA from bacterial cell and its digestion

CO82 : Design, modify and apply the working of PCR to research.

Discussion, Tutorials, Demonstration, Effective questions, Simulation, Giving tasks, Practical

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

1.    Agarose gel electrophoresis

2.    Gene Transfer-Conjugation

3.    Transformation of E.coli

4.    Preparation of competent cells

5.    Bacteriophage Plaque Assay for Phage Titer

6.    Isolation of plasmid DNA from bacterial cell.

7.    Restriction Digestion of Lambda DNA

8.    Demonstration of PCR

9.    Demonstration of SDS-PAGE 

Academic Year: 
2023-2024 [2]

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Source URL: https://biotech.iisuniv.ac.in/courses/subjects/laboratory-viii-0

Links:
[1] https://biotech.iisuniv.ac.in/courses/subjects/laboratory-viii-0
[2] https://biotech.iisuniv.ac.in/academic-year/2023-2024