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Laboratory VIII
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO77: Understand and relate to various genetic engineering techniques. CO78: Use and illustrate various gene transfer techniques like conjugation and transformation CO79: Plan to prepare competent cells CO80: Pivot after understanding the principle underlying Gel electrophoresis and SDS page CO81: Appraise knowledge to isolate Plasmid DNA from bacterial cell and its digestion CO82 : Design, modify and apply the working of PCR to research. |
Discussion, Tutorials, Demonstration, Effective questions, Simulation, Giving tasks, Practical
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Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
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1. Agarose gel electrophoresis
2. Gene Transfer-Conjugation
3. Transformation of E.coli
4. Preparation of competent cells
5. Bacteriophage Plaque Assay for Phage Titer
6. Isolation of plasmid DNA from bacterial cell.
7. Restriction Digestion of Lambda DNA
8. Demonstration of PCR
9. Demonstration of SDS-PAGE
