INSTRUMENTATION

Paper Code: 
MBL 124
Credits: 
05
Contact Hours: 
75
Objective: 

Course Objectives:

This course will enable the students to –

  1. Understand the basic operating characteristics of instruments.
  2. Understand principles and applications of techniques.

Course Outcomes (COs): 

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 124

 

Instrumentation

 

Upon completion of the course students will be able to

CO 15:Evaluate the basic operating, characteristics of bioinstruments.

CO 16:Assess various methods of spectroscopy and chromatography

and electrophoresis.

CO 17: Evaluate centrifugation methods and utility of different types of microscopy

CO 18: Assess various radioisotopic techniques

Approach in teaching:

Discussion, Demonstration, Project, Field Trip, Learning activities for the students:

Self-learning assignments, Effective questions, Presentation, Giving tasks

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

 

17.00
Unit I: 
Spectroscopy

Beer-Lambert law, UV-Vis spectroscopy, fluorescence spectroscopy, IR spectroscopy, Raman spectroscopy, Atomic absorption spectroscopy, Mass spectroscopy, NMR, ESR, CD/ORD, MALDI-ToF-MS, X-Ray Diffraction.

15.00
Unit II: 
Chromatography

Principle, Paper chromatography, TLC, Column chromatography (GC, HPLC), Adsorption chromatography, Partition chromatography, Gel filtration, Ion-exchange chromatography and Affinity chromatography.

15.00
Unit III: 
Electrophoresis

Principle, factors affecting electrophoresis, pH, voltage, supporting medium (agarose, polyacrylamide, dextran).

Agarose gel electrophoresis, PAGE, SDS-PAGE, 2-D electrophoresis, Pulsed field gel electrophoresis, isoelectric focusing, immuno electrophoresis.

20.00
Unit IV: 
Centrifugation & Microscopy

Centrifugation: principle differential and density gradient, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF.

Microscopy: construction of a microscope, light microscopy (bright field, dark field, phase-contrast, interference, confocal, polarization microscopy).

Electron microscopy-TEM, SEM, Scanned probe microscopic techniques (STEM, AFM)

Cytophotometry, flow cytometry.      

8.00
Unit V: 
Radioisotopic Techniques

Radioisotopes; nature of radioactivity, types of radioactive decay, unit of radioactivity.

Radiation dosimetry, radioactive isotopes, autoradiography, Cerenckov radiation, liquid scintillation counting, Geiger-Muller counter.

ESSENTIAL READINGS: 
  1. Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
  2. Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou,  Fitzgerald science press, Inc., 1998
  3. Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003
  4. Principles and Techniques  of Biochemistry and  Molecular Biology ,(6th edition), K Wilson and J Walker (editor), Cambridge University Press, 2007
REFERENCES: 
  1. Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004    
  2. Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  3. Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2004
  4. Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005  
Academic Year: 
2020-2021
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