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Animal Cell Science and Technology
This paper will enable students to- • understand the basics of Animal Cell Sciences • learn the basic requirements for Animal Cell Culture • learn the various types of Animal Cell Culture and techniques used in Animal Cell Culture • study the commercial aspect of Animal Biotechnology
Structure, organization and biology of animal cell
General concepts of animal cell culture
Proliferation, differentiation and dedifferentiation
Expression of proteins in animal cells
Cell Culture laboratory
Media -Development of simple growth media, BSS -Chemical, physical and metabolic functions of different constituents of culture medium -Role of CO2 and serum Serum and protein free defined media and their applications Growth kinetics of cultured cells
Primary and established cell line cultures Cell lines: A general concept Disaggregation of tissue and primary culture Cell separation Organ and histotypic cultures
Cell cloning and micromanipulation Transfection techniques Cell transformation Scaling-up of animal cells
Cell culture based vaccines Stem cell culture, Embryonic stem cells and their applications Cell hybridization, Hybridomas and their selection, application of cell hybridization
1. Culture of Animal Cells by R. Ian Freshney, 4th edition, Wiley-Liss, 2000 2. Animal Cell Culture-Practical Approach by John R.W. Masters, 3rd edition, Oxford Press, 2000
1. Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000 2. Animal Cell Culture Methods, Jennie P. Mather and David Barnes, Vol 57, Academic Press, 1998 3. Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004 4. Principles of Gene Manipulation, S.B. Primrose, R.M. Twyman and R.W. Old, 6th edition, Blackwell Science, 2003
