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Genetic Engineering
This paper will enable students to- • learn the basics of Genetic Engineering • study different cloning and expression vectors • study the enzymes used in Genetic Engineering • learn the procedures involved in isolation of genomic and nuclear DNA
Milestones & Importance of Genetic Engineering, vehicles for gene cloning: Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC.
Introduction of foreign DNA into plant and animal cells.
Enzymes used in G.E: DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase, Reverse transcriptase, Restriction endonucleases, Nick translation system.
Techniques: PCR, Colony and Plaque hybridization, Northern, southern, Western hybridization, Dot and slot Blot technique, DNA sequencing: Maxam Gilbert method, Sanger’s method, automated DNA sequencing
Production of protein from cloned genes, recombinant insulin, growth hormones, human proteins, recombinant vaccines, genetic counseling.
In Agriculture: Insect resistance, Virus resistance, modification of seed protein quality, suppression of endogenous genes, male sterility, biochemical production, plant derived vaccines, In Forensic science: identification of crime suspects, kinship analysis
1. S.B.Primorse, (1994) Molecular Biotechnology (2nd Edn.), Blackwell Scientific Publishers, Oxford. 2. M.R.Walker and R.Repley, (1997). Route Maps in Gene Technology, Blackwell Science Ltd.Oxford. 3. T.A. Brown ,(2001). Gene Cloning and DNA Analysis. An Introduction. Blackwell Science.
1. Sambrook J., Fritsch E.F and Maniatis T. (2000) Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory Press, New York. 2. Glover D.M. and Hames B.D. (1955) DNA Cloning: a practical Approach, , IRL Press Oxford. 3. Kaufman P.B., Kim W.Wu. D.and Cseke L.J, (1995). Molecular and Cellular Methods in Biology and Medicine, CRC Press. Florida,. 4. Berger S.L. and Kimmel A.R. (1998). Methods in Enzymology, Guide to Molecular Cloning Techniques, Academic press. San Diego, 5. Gooddol D.V. (1990). Methods in Enzymology, Gene Expression Technology, Academic Press, San Diego. 6. Mickloss D.A.and Greyer G.A. (1990). DNA Science. A First Course in Recombinant Technology, ,Cold Spring Harbor Laboratory Press, New York, 7. Davies J.A and Roznikolf W.S. (1992). Milestones in Biotechnology. Classic papers on genetic Engineering, Butterworth-Helnemann, Boston,. 8. Kingsman S.M. and Kingsman A.J (1998). Genetic Engineering. An Introduction to gene analysis and exploitation in eukaryotes. Blackwell Scientific Publications, Oxford, 1998.
