Laboratory I

1. Preparation of liquid and solid media for growth of microorganisms. 2. Pure culture techniques- streak plate method, pour plate method, spread plate method. 3. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth. 4. Enumeration of bacteria: standard plate count. 5. Microscopic examination of bacteria by simple staining, Gram stain, Negative staining, staining for spores etc. 6. Hanging drop method to study motility in bacteria. 7. study of culture characters of microorganisms 8. Biochemical characterization of selected microbes. a) oxidation and fermentation tests b) catalase test c) oxidase test d) nitrate reduction test e) starch hydrolysis f) IMVIC g) H2S production test 9. Estimation of protein in bacteria by Folin Lowry method 10. Estimation of protein by Biuret test 11. Estimation of reducing sugar by DNSA method 12. Estimation of sugar in bacteria by Anthrone reagent 13. To study the effect of substrate concentration on enzyme activity 14. To study the effect of temperature on enzyme activity 15. To study the effect of pH on enzyme activity 16. To assay the activity of acid phosphatase 17. Separation and identification of amino acids by TLC 18. Separation of protein by SDS-PAGE 19. Demonstrations: a. Gel Filtration Chromatography b. Ion Exchange Chromatography c. Adsorbtion Chromatography d. Affinity Chromatography e. Blotting Techniques 20. Biostatistics exercises 21. Information searching on Internet. 22. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ Any other practical based on theory syllabus.