Laboratory II

1. Isolation of antibiotic resistant microorganisms by replica plating. 2. Isolation of antibiotic resistant microorganisms by Gradient plate technique. 3. Effect of U.V. rays on bacteria. 4. Isolation of plasmid DNA 5. Isolation of genomic DNA 6. Spectrophotometric quantitation of nucleic acids 7. Ouchterlony double diffusion 8. ELISA 9. Demonstrations: a. Sandwich ELISA b. Latex agglutination c. Rocket Immunoelectrophoresis 10. Restriction digestion of λDNA 11. Ligation 12. Transformation of DH5α (including preparation of competent cells) 13. Demonstartions: d. Conjugation e. Reporter gene assay (GFP)/ Bacterial Transduction 14. Study of various symptoms produced in plants due to virus infection. 15. Study of viral diseases of plants/animals/human (Specimen/photographs) 16. Different type of viruses (Photographs/sketches). 17. Titration of Phages 18. Isolation and identification of fungi . 19. study of permanent slides of algae and fungi 20. Isolation and identification of Rhizobium from root nodules of leguminous plants. 21. Isolation and identification of Azotobacter from soil.