Laboratory -III

Paper Code: 
BTE 303
Credits: 
02
Contact Hours: 
60
Objective: 

This course will enable the students to –

 

  1.  Learn to differentiate between genomic, plasmid DNA and RNA.
  2. Learn the concept of Mendelian genetics
  3. Learn pure culture techniques for fungi.

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 303

Laboratory III

CO 39 Student will learn to culture and identify protozoa.

 

CO 40 Student will learn to isolate and genomic DNA, Plasmid DNA as well as RNA.

 

CO 41 Student will learn Mendelian genetics

 

CO 42 Student will learn to analyse and solve problems based on Mendeliangentics

 

CO 43 Student will learn pure culture techniques for fungi.

 

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction
 

 

CONTENTS

 

  1. Isolation of mutants by replica plating.
  2. Isolation of mutants by gradient plate technique.
  3. Simple problems based on Mendel’s laws and gene interactions.
  4. Isolation of genomic DNA from bacterial cell.
  5. Isolation of DNA from plant cell.
  6. Estimation of DNA by Gel electrophoresis
  7. Determination of λmax from isolated DNA
  8. Isolation  of RNA
  9. Antibiotic Sensitivity Test

 

Academic Year: 
2020-2021
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