Department Events
No Upcoming Events
Molecular Biology and Microbial Genetics
This paper will enable students to: • Understand the basic mechanism involved in the flow of genetic information • Understand molecular mechanism of mutations and DNA repair • Understand recombination in bacteria • Have an idea of cloning vectors
DNA as a genetic material, structure of DNA and RNA, Types of DNA (A, B, Z), DNA replication in prokaryotes and eukaryotes.
Mechanism of Transcription in prokaryotes and eukaryotes, Enzymes and transcription factors, Posttranscriptional modifications of eukaryotic mRNA.
The genetic code, Translational machinery in eukaryotes and prokaryotes, inhibitors of protein synthesis
Regulation of Gene expression in prokaryotes (lac and trp operon)
Molecular mechanism of mutation, forward and reverse mutation, transition, transversion, Spontaneous and induced mutations
Methods for mutant selection- direct selection, antibiotic selection, replica plating
Mechanism of DNA repair-photo reactivation, Nucleotide excision repair, mismatch repair
Transformation, transduction and conjugation
A brief idea of cloning vectors (plasmids, cosmids, bacteriophages).
1. Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2005. 2. Gene VI, Lewin, B, New York, Oxford University Press, 2000. 3. Cell and Molecular Biology, 4th edition, Gerald Karp, John Wiley and Sons. 2005.
4. Biochemistry and Molecular Biology, 3rd edition, Elliott W H and Elliot D C, Oxford University Press, 2005. 5. Essentials of Molecular Biology, 3rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 1998. 6. Instant Notes, Molecular Biology. Turner P.G., Mc Lennan, Bates A D, and White M R H, 2nd edition. Bios Scientific Publishers. 2002. 7. Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons. 1987. 8. Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 1994.
