Molecular Biology

Paper Code: 
BIF 221
12.00
Unit I: 
Basics of Molecular Biology

Structure and properties of nucleic acids, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization, DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication. Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.

14.00
Unit II: 
Central dogma of molecular biology

Transcription in prokaryotes and eukaryotes, Transcription factors, regulation of transcription. Genetic code, Translational machinery, mechanisms of initiation, elongation and termination, regulation of translation, Co and post- translational modifications of proteins.

10.00
Unit III: 
Gene Mutations & Chromosomal aberrations

Molecular basis of mutation, spontaneous & induced mutations, chemical and physical mutagens, Ames test. DNA repair mechanisms-photo reactivation, excision repair, mismatch repair, recombination repair, and SOS response.

12.00
Unit IV: 
Signal transduction and cell cycle

Molecular basics of signal transduction in bacteria, plants and animals, secondary messengers, perception of environmental signals, G- protein and tyrosine kinase coupled receptors. Cell cycle and apoptosis: Events in cell cycle, mitosis and meiosis, Key regulators of cell cycle, programmed cell death and its control. Oncogenesis

12.00
Unit V: 
Immunology

Immunity; basic types, Cells of immune system, Antigen and it’s properties, antibodies & it’s types, effector mechanisms.

ESSENTIAL READINGS: 

1. J. Sambrook, E. F. Fritsch and I. Maniatis. “Molecular Cloning: a Laboratory Manual”.Cold Spring harbor Laboratory Press, New York,2000. 2. P. D. Dabre. “Introduction to Practical Molecular Biology”, John Wiley & Sojns Ltd.,Yourk,1988. 3. T.A. Brown Genomes. John Wiley and sons(ASIA) PTE Ltd 1999 4. B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson. “Molecular Biology of the Cell”. 2nd Edition, Garland publishing. Inc., New York, 1994. 5. Watson, Baker, Bell, Gann, Levin, Losick. “Molecular Biology of the Gene”. 5th Edition, Pearson Education,2003. 6. J. Darnell, H. Lodish and D.Baltimore. “Molecular Cell biology”.2nd Edition, Scientific American Books, USA, 1994. 7. G M Malacinski, D Freifelder. “Essentials of Molecular Biology”.Jones & Bartlett Publishers Inc.,1998. 8. Winnacker “From Genes to Clones” Panima Publishing Corporation, New Delhi/Bangalore, 2003

REFERENCES: 

1. Russell, Peter. J. “Genetics – A molecular approach”, 2003. 2. Benjamin Lewin. “Gene IX”. 9th Edition. Oxford University Press.U.K., 2007. 3. R. A. Meyers. “Molecular Biology and biotechnology. A comprehensive desk reference”. VCH Publishers, Inc., New York, 1995.

Academic Year: 
2018-2019
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