Molecular Biology

Paper Code: 
BTE 222
Credits: 
5
Contact Hours: 
75
Objective: 

to enable students to-

  • understand the central dogma of molecular biology.
  • understand process of prokaryotic and eukaryotic replication.
  • know machinery involved in Prokaryotic and eukaryotic transcription.
  • get a detailed insight of mechanism and regulation of prokaryotic and eukaryotic translation process.
  • study the molecular mechanisms of antisense and ribozyme technology.
10.00
Unit I: 
Prokaryotic and Eukaryotic gene structure and function.

Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization

15.00
Unit II: 
Replication in Prokaryotes and Eukaryotes.

DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.

15.00
Unit III: 
Transcription in Prokaryotes and Eukaryotes

Prokaryotic & Eukaryotic transcription (Initiation ,Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability.

15.00
Unit IV: 
Machinery involved in Prokaryotic and Eukaryotic Translation.

Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.

20.00
Unit V: 
Antisense and Ribozyme Technology.

Molecular mechanism of antisense molecules-Inhibition of splicing, polyadenylation, translation. disruption of RNA structure & capping, Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb & p53 tumor suppressor proteins.

ESSENTIAL READINGS: 

1. Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2000. 2. Introduction to Practical Molecular Biology, P. D. Dabre, John Wiley & Sojns Ltd.,Yourk,1988. 3. Genomes. Brown T.A: John Wiley and sons(ASIA) PTE Ltd1999 4. Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994. 5. Molecular Biology of the Gene. (5th Edition) Watson, Baker, Bell, Gann, Levin, Losick: Pearson Education.2003. 6. Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994. 7. Essentials of Molecular Biology. Malacinski, G M, Freifelder D: Jones & Bartlett Publishers Inc. (1998) 8. From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003

REFERENCES: 

1. Genetics – A molecular approach by Russell, Peter. J, 2003 2. Gene IX (9th Edition) Benjamin Lewin. Oxford University Press.U.K., 2007 3. Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.

Academic Year: 
2018-2019
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