Animal Cell Science and Technology

Paper Code: 
BTE 421
Credits: 
5
Contact Hours: 
75
Objective: 

pagesto enable students to-

  • learn the basics of Animal cell culture.
  • know the various growth requirements and concept of differentiation of animal cells in vitro conditions.
  • become acquainted with the techniques for manipulating the cells lines in laboratory conditions.
  • know the applications of animal cell culture.
15.00
Unit I: 
Introduction to animal cell culture

Structure, organization and biology of animal cell, Principles of animal cell culture, need to express in animal cell, Equipments and materials for animal cell culture technique General concept of differentiation and dedifferentiation

15.00
Unit II: 
Growth requirements and kinetics:

Media Development of simple growth media , Introduction to balanced salt solution, Chemical, physical and metabolic functions of different constituents of culture medium, Role of CO2, Role of serum supplements, Serum and protein free defined media and their applications, Biology and characterization of cultured cells, measuring parameters of growth.

15.00
Unit III: 
Animal cell culture and its types

Primary and established cell line cultures, Cell lines- Introduction, diversity and selection, nomenclature, routine maintenance and cryopreservation, Disaggregation of tissue and primary culture, Cell separation, , Organ and histotypic cultures.

15.00
Unit IV: 
Characterization and manipulation of cells,

Measurement of viability and cytotoxicity, Cell synchronization, Cell cloning and micromanipulation, Cell transformation, Scaling-up of animal cells, Measurement of cell death, Apoptosis.

15.00
Unit V: 
Applications of animal cell culture

Three dimensional culture and tissue engineering, Application of animal cell culture, Stem cell culture, embryonic stem cells and their applications, Cell culture based vaccines, Somatic cell genetics, Cell hybridization, Hybridomas and their selection, application of cell hybridization.

ESSENTIAL READINGS: 

1. Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley-Liss. 2000 2. Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000

REFERENCES: 

1. Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998 2. Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000 3. Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004 4. Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003

Academic Year: 
2018-2019
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