Laboratory I

1. Microtomy of liver/intestine 2. Histochemical localistion of starch, lipids and protein in liver. 3. Verification of Beer Lambert’s Law 4. Estimation of protein by Folin Lowry method 5. Estimation of protein by Biuret test 6. Estimation of reducing sugar by DNSA method 7. Estimation of sugar by Anthrone reagent 8. Estimation of glycogen from the given tissue 9. Estimation of cholesterol from the given tissue 10. To study the effect of substrate concentration on enzyme activity 11. To study the effect of temperature on enzyme activity 12. To study the effect of pH on enzyme activity 13. To assay the activity of acid phosphatase 14. Separation and identification of amino acids by TLC 15. Separation of protein by SDS-PAGE 16. Demonstrations: a. Gel Filtration Chromatography b. Ion Exchange Chromatography c. Descending Paper Chromatography d. Column Chromatography e. Affinity Chromatography f. Western Blotting 17. Preparation of liquid and solid media for growth of microorganisms. 18. Isolation and maintenance of organisms by plating, streaking and serial dilution methods, slants and stab cultures, storage of microorganisms. 19. Isolation of pure cultures from soil and water. 20. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth. 21. Enumeration by standard plate count. 22. Microscopic examination of bacteria, yeast and molds and study of organisms by Gram stain, Negative staining, staining for spores etc. 23. Isolation of Rhizobia 24. Isolation of Actinomycetes 25. Biochemical characterization of selected microbes. 26. Biostatistics exercises 27. Operating systems (DOS) 28. Information searching on Internet. 29. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ Any other practical based on theory syllabus.