Laboratory II

1. Study of mitosis and meiosis. 2. X – Chromosome detection through Barr body experiment. 3. To study mitosis in onion root tips 4. To study meiosis in onion buds 5. Isolation of plasmid DNA 6. Isolation of genomic DNA 7. Spectrophotometric quantitation of nucleic acids 8. Demonstration of lac operon 9. Blood film preparation and identification of cells. 10. Ouchterlony double diffusion 11. Dot ELISA 12. Demonstrations: a. Sandwich ELISA b. Latex agglutination c. Rocket Immunoelectrophoresis 13. Restriction digestion of ?DNA 14. Ligation 15. Transformation of DH5a (including preparation of competent cells) 16. Demonstartions: a. Conjugation b. Reporter gene assay (GFP) 17. Preparation of media. 18. Explant inoculation 19. Callus induction 20. Protoplast isolation and fusion 21. Synthetic seed preparation 22. Study of mutagens by Ames test. 23. Blood film preparation and identification of cells.

Any other practical based on theory syllabus