Analytical Techniques

Paper Code: 
AMB 102
Credits: 
03
Contact Hours: 
45
Objective: 

 

This paper will enable students to-
  • learn various techniques used in Biotechnology
  • understand general concepts of Mathematics as applied to Biology
9.00
Unit I: 
Microscopy

Introduction to basic principle and applications:Light microscopy (bright field, dark field, phase contrast, interference, fluorescence microscopy), electron microscopy (TEM and SEM), Confocal Microscopy

9.00
Unit II: 
Chromatography

Principle of Paper Chromatography, Thin Layer Chromatography, Column Chromatography (GC, HPLC), Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography

8.00
Unit III: 
Centrifugation

Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF

10.00
Unit IV: 
Electrophoretic Techniques

 

Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran).
Agarose gel electrophoresis, PAGE, SDS-PAGE. Zone electrophoresis, Isoelectric focussing, Immuno-electrophoresis
9.00
Unit V: 
Spectroscopy

 

Introduction to basic principles and applications:
Spectrophotometry (UV-visible, infra red, AAS), Fluorimetry, NMR, Mass spectroscopy
ESSENTIAL READINGS: 

 

1. Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
2. Principles and Techniques  of Biochemistry and  Molecular Biology, 6th edition,  K Wilson and J Walker (ed.) Cambridge University Press, 2007
REFERENCES: 

 

1. Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004     
2. Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
3. Spectroscopy for the Biological Sciences, G.G Hames, John Wiley & Sons Inc. 2005   
Academic Year: 
2018-2019
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