Department Events
No Upcoming Events
Fundamentals Of Microbiology
Paper Code:
AMB 201
Credits:
03
Contact Hours:
45
Objective:
This paper will enable students to:
- have an insight in to history of microbiology
- understand classification and characters of microorganisms.
- learn the techniques used for isolation and staining of microbes.
- have an insight in growth and preservation of microorganisms.
8.00
Unit I:
History of Microbiology
Introduction, discovery of microbial world, experiments of Pasteur, the era of discovery of antibiotics, discovery of anaerobic life, physiological significance of fermentation.
Isolation methods for microorganisms. Culture collections.
8.00
Unit II:
Microbial Diversity-I
Concept of culturable and non culturable microorganisms.
Anaerobic ecosystem- rumen microbiology
Characteristics, ultra structure and classification of bacteria (morphological, nutritional and biochemical).
9.00
Unit III:
Microbial Diversity-II
Classification, characteristics and structure of: Viruses, Mycoplasma, Actinomycetes, Fungi and Algae.
11.00
Unit IV:
Structural Components and Staining Techniques
Structure of flagella, capsule, slime layer, inclusion bodies like glycogen granules, volutin granules etc., endospores and plasmids.
Stains and staining techniques: Principles of staining, Types of stains – simple stains and differential stains.
9.00
Unit V:
Growth and preservation of microbes
A brief idea of microbial growth (different phases of growth).
Factors affecting growth. Preservation of microbes by serial subculture, mineral oil and lyophilization. Other methods for storage of fungi.
ESSENTIAL READINGS:
1. General Microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
2. General Microbiology, CB Powar, H F Daginawala, Himalayan Publication House, 1996
3. Microbiology ,5th edition M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2006
4. Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006
REFERENCES:
1. Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
2. General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
3. Laboratory fundamentals of microbiology, I E Alcamo, Jones and Barlett Publishers, 2001
4. Microbiology , 6th edition, Prescott, Harley, Klein, Mc Graw Hill Companies, 2005
5. Microbiology Fundamentals and Applications ,2nd edition, R M Atlas, Maxwell Macmillan International Edition, 1989
Academic Year:
