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Tissue Culture
Paper Code:
AMB 402
Credits:
03
Contact Hours:
45
Objective:
This paper will enable students to-
- learn the fundamental of Plant Tissue Culture
- get acquainted with various techniques in Plant Tissue Culture
- analyze the genetic manipulations in Plant Tissue Culture
- study the industrial application of Plant Tissue Culture
- understand the basics of Animal Cell Sciences
- learn the basic requirements for Animal Cell Culture
12.00
Unit I:
Introduction to Plant Tissue Culture
History Laboratory Requirements and General Techniques: Introduction, Techniques and Requirements.Tissue Culture Media: Introduction, medium constituents, media Selection, Media preparation.Cell Culture: Introduction, isolation of single cells, suspension cultures, Culture of single cells, plant cell reactors, application of cell culture
Cellular Totipotency: Cytodifferentiation, Organogenesis Differentiation. Application of cellular totipotency.
8.00
Unit II:
Embryogenesis
Somatic Embryogenesis: Introduction, Induction, development and maturation of Somatic Embryos Large Scale production of Somatic Embryos. Somatic Embryo versus Zygotic Embryos. Synthetic seeds. Applications of Somatic Embryogenesis
In – vitro Pollination and fertilization: Introduction, terminology, In vitro pollination,
In vitro fertilization, applications.
10.00
Unit III:
Genetic Engineering in Plants
Genetic transformation of plants: Introduction, methods of Gene transfer, selection and identification of transformed cells, Applications of genetic transformation.
Germplasm Storage: Cryopreservation, Introduction, Long – term storage, Short term or medium – term storage.
Production of Secondary Metabolites: Strategies to optimize product yield, commercial aspects.
7.00
Unit IV:
Introduction and Growth Requirements for Animal Cell Culture
General concepts of animal cell culture, Serum free media and role of serum in serum media, Growth Kinetics
8.00
Unit V:
Culture of Animal Cells
Primary and established cell line cultures, Concept of differentiation and dedifferentiation
Transfection methods, Scaling up of animal cells.
ESSENTIAL READINGS:
1. Advanced Methods in Plant Biotechnology, David R Murray, Panima Publishing Corporation. 1996
2. Plant, Genes and Crop Biotechnology, 2nd Edition, Marten J Chrispeels & David E Sadava, Jones and Barlett Publishers. 1999.
3. Biotechnology in Crop Improvement, H S Chawla, International Book Distributing Company, 1998.
4. Practical Application of Plant Molecular Biology, R J Henry, Chapman and Hall,1997.
5. Laboratory Manual for Plant Biotechnology, H S Chawla Oxford & IBH publishing Co. Pvt Ltd. 2003
6. Plant Biotechnology – The Genetic Manipulation of Plants, Adrian Slater, Nigel Scott & Mark Fowler, Oxford University Press. 2003
7. Plant Tissue Culture – Techniques & Experiments, 2nd edition, Roberta, H Smith, Elsevier, 2000
8. Culture of Animal Cells, 4th edition, R Ian Freshney, Wiley-Liss, 2000
9. Animal Cell Culture-Practical Approach, 3rd edition, John R.W. Masters, Oxford, 2000
REFERENCES:
1. Molecular Plant Biology- Volume 1 & 2. Philip M Gilmartin and Chris Bowler: Oxford University Press. 2005
2. Plant Tissue Culture, S S Bhojwani and M.K Razdan, Elsevier, 2004
3. Plant Biotechnology, J Hammond, P Mc Garvey & V Yusibov, Springer (India) Pvt Ltd. 2000
4. Plant Cell and Tissue Culture for the Production of Food ingredients, J Fu, G Singh, and W R Curtis (Eds), Kluwer Academic/Plenum Press, 1999.
5. Biotechnology – Secondary Metabolites. K G Ramawat, J M Merllon, Oxford & IBH publishing house Co. Pvt. Ltd. 2000
6. Plant Molecular Biology – A Practical Approach, C H Shaw, IRL Press Ltd. 1988.
7. Basic Cell Culture by J M Davis, Oxford , 2000
Academic Year:
