Advance Paper-I Paper III: Biotechnology and Bioinformatics

Paper Code: 
25LSC143A
Credits: 
04
Contact Hours: 
60

UNITS

TOPICS

TEACHING HOURS

Unit I

RECOMBINANT DNA TECHNOLOGY:

Restriction endonucleases and methylases; DNA ligase, Klenow enzyme, T4 DNA polymerase, polynucleotide kinase, alkaline phosphatase; cohesive and blunt end ligation;
linkers; adaptors; homopolymeric tailing; labelling of DNA: nick translation, Plasmids PUC19; Bacteriophages M13; Cosmids; Artificial chromosome vectors (YACs; BACs); Principles for maximizing gene expression expression vectors- GST; pET-based vectors; Protein purification- His-tag, GST-tag; Intein-based vectors; Inclusion bodies; mammalian expression vectors; Baculovirus vector, Ti as vectors; shuttle vectors

 

12

Unit II

RNA – BASED TECHNOLOGIES

Principle; Antisense oligonucleotides; siRNA; miRNA; lncRNAs; RNAi in plants and fungi; application of RNAi for studying gene expression; cDNA synthesis; RT-PCR; QPCR; Nanobiotechnology based RNA for therapeutics/ diagnosis/ imaging

 

12

Unit III

ENVIRONMENTAL BIOTECHNOLOGY:

Identifying new genes with metagenomics, culture enrichment for environmental samples, sequence-dependent techniques for metagenomics, Function or activity-based evaluation of the environment. Ecology and metagenomics, Natural attenuation of pollutants

 

12

Unit IV 

Bioinformatics

Molecular databases- primary and secondary databases, Sequence alignment techniques, scoring systems – dynamic programming, similarity searching using BLAST and FASTA. EMBOSS tools for DNA and protein sequence analysis

 

12

Unit V 

Phylogenetics

MSA and phylogenetic analysis, methods of phylogenetic tree construction, Bootstrapping technique protein structure analysis and modelling

 

12

 

ESSENTIAL READINGS: 
  • Plant, Gene and crop Biotechnology, M.J. Chrispeel and D.E. Sadava ASPB 2003.
  • Textbook of Environmental Biotechnology, P.K Mohapatra, I K International Publications, Mumbai 2006.
  • Environmental Chemistry, A.K.De, Willey Eastern Ltd., New Delhi.
  • Microbial Ecology- Fundamentals and applications. Ronald. M. Atlas, Richard Bartha. Pearson Education. 2005.
  • Advances in Industrial Waste water treatment. P.K. Goel. (First Edition).Technosound Publications. 1999.
  • Wastewater Engineering- Treatment, Disposal and Resuse, Metcalf and Eddy,Inc., Tata McGraw Hill, Delhi.1999
  • Comprehensive Biotechnology, vol.4, M. Moo- Young (Ed-in-chief), Pergamon Press, Oxford.
  • Introduction to Biodeterioration. D. Allsopp and K.J. Seal, ELBS/Edward Arnold.
  • Bioremediation Engineering: design and Application. Cookson, J.T. McGraw-Hill, Inc.1995
  • Environmental Biotechnology – Basic concepts and applications, Indu Shekhar
  • Thakur, I K International publications. 2006 Molecular Cloning: a Laboratory
  • Manual, J. Sambrook. E.F. Fritsch and T. Maniatis, Cold Spring HarborLaboratory Press, New York, 2000
  • DNA Cloning: a practical Approach, D. M. Glover and B.D. Hames, IRL Press Oxford, 1955.
  • Molecular and Cellular Methods in Biology and Medicine, P. B. Kaufman, W. Wu., D. Kim and L.J: Cseke, CRC Press.Florida,1995.
  • An Introduction to Genetic Engineering. Edited by Desmond S.T. Nicholl,Cambridge University Press, February 2002.
  • Manipulation and Expression of Recombinant DNA. Sue Carson and Dominique
  • Robertson, Second edition, Academic Press, December 2005.
  • Principles of Gene Manipulation and Genomics. Primrose and Twyman, (7thedition). Blackwell Publishers, 2006.
  • RNA interference Technology- From basic science to drug development. Edited by Krishnarao Appasani. Forewords by Andrew Fire and Marshall Nirenberg,Cambridge Press, 2005.
  • Methods in Enzymology Vol.152,Guide to Molecular Cloning Techniques, S. L.Berger and A. R. Kimmel, Academic press. Inc. San Dlogo, 1998.
  • Methods in Enzymology Vol.185,Gene Expression Technology, D. V. Gooddol, Academic Press, Inc., San Diego,1990.
  • DNA Science. A First Course in Recombinant Technology, D.A. Mickloss and G.A. Greyer, Cold Spring Harbor Laboratory Press, New York,1990
  • Molecular Biotechnology (2nd Edition), S.B. Primorse, Blackwell ScientificPublishers, Oxford, 1994.
  • Milestones in Biotechnology. Classic papers on genetic Engineering, J.A. Davies and W.S.Roznikolf, Butterworth-Helnemann, Boston, 1992.
  • Route Maps in Gene Technology, M. R. Walker and R.Repley, Blackwell Science Ltd. Oxford, 1997.
  • Genetic Engineering. An Introduction to gene analysis and exploitation in
  • Eukaryotes. S. M. Kingsman and A.J. Kingsman, Blackwell Scientific Publications. Oxford, 1998.
  • Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids. S Nimesh, Nidhi Gupta, R Chandra.Woodhead Publishing Series in Biomedicine, Oxford, UK, 2017
  • Cancer Therapy: A Potential Application of Nanotechnology. S Nimesh, Nidhi Gupta, Guilherme Carneiro, Elsevier Publications, May 2024
  • Thakur, I K International publications. 2006 Molecular Cloning: a Laboratory
  • Manual, J. Sambrook. E.F. Fritsch and T. Maniatis, Cold Spring HarborLaboratory Press, New York, 2000
  • DNA Cloning: a practical Approach, D. M. Glover and B.D. Hames, IRL Press Oxford, 1955.
  • Molecular and Cellular Methods in Biology and Medicine, P. B. Kaufman, W. Wu., D. Kim and L.J: Cseke, CRC Press.Florida,1995.
  • An Introduction to Genetic Engineering. Edited by Desmond S.T. Nicholl,Cambridge University Press, February 2002.
  • Manipulation and Expression of Recombinant DNA. Sue Carson and Dominique
  • Robertson, Second edition, Academic Press, December 2005.
  • Principles of Gene Manipulation and Genomics. Primrose and Twyman, (7thedition). Blackwell Publishers, 2006.
  • RNA interference Technology- From basic science to drug development. Edited by Krishnarao Appasani. Forewords by Andrew Fire and Marshall Nirenberg,Cambridge Press, 2005.
  • Methods in Enzymology Vol.152,Guide to Molecular Cloning Techniques, S. L. Berger and A. R. Kimmel, Academic press. Inc. San Dlogo, 1998.
  • Methods in Enzymology Vol.185,Gene Expression Technology, D. V. Gooddol, Academic Press, Inc., San Diego,1990.
  • DNA Science. A First Course in Recombinant Technology, D.A. Mickloss and G.A. Greyer, Cold Spring Harbor Laboratory Press, New York,1990
  • Molecular Biotechnology (2nd Edition), S.B. Primorse, Blackwell Scientific Publishers, Oxford, 1994.
  • Milestones in Biotechnology. Classic papers on genetic Engineering, J.A. Davies and W.S.Roznikolf, Butterworth-Helnemann, Boston, 1992.
  • Route Maps in Gene Technology, M. R. Walker and R.Repley, Blackwell Science Ltd. Oxford, 1997.
  • Genetic Engineering. An Introduction to gene analysis and exploitation in
  • Eukaryotes. S. M. Kingsman and A.J. Kingsman, Blackwell Scientific Publications. Oxford, 1998.

 

Academic Year: 
2025-2026
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