ANALYTICAL TECHNIQUES

Paper Code: 
MBL 102
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

 

  1. Learn various techniques used in Biotechnology for experimentation.
  2. The students will be able to understand the basic principles and working of different            instruments present in a Biotechnology lab and their applications.

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 102

Analytical Techniques

CO 1 Explain the various separation techniques and its instrumentation.

CO 2 Understand various fundamentals of spectroscopy, qualitative and quantitative analysis and characterize functionalities of biomolecules by using spectroscopic techniques.

CO 3 Understand Principle, working, ray diagram and application of advance microscopes

CO 4 Understand different separation techniques

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

 

C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction

 

9.00
Unit I: 
Microscopy

Introduction to basic principle and applications: Light microscopy (bright field, dark field, phase contrast and fluorescence microscopy), electron microscopy (TEM and SEM)

10.00
Unit II: 
Optical methods of Analysis

Introduction to basic principles and applications: Beer Lambert’s Law Spectrophotometry (UV-visible, infra red, AAS), Mass spectroscopy

8.00
Unit III: 
Chromatography

Working principle, instrumentation and application of:  Paper Chromatography, Thin Layer Chromatography, Column Chromatography (Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography)

 

10.00
Unit IV: 
Electrophoretic Techniques

Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran), Agarose gel electrophoresis, PAGE, SDS-PAGE, isoelectric focusing

 

8.00
Unit V: 
Centrifugation

Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (preparative and analytical centrifuges) sedimentation analysis & RCF          

 

ESSENTIAL READINGS: 
  1. Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  2. Principles and Techniques  of Biochemistry and  Molecular Biology, 6th edition,  K Wilson and J Walker (ed.) Cambridge University Press, 2007

 

REFERENCES: 

1.      Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004    

2.      Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000

3.      Spectroscopy for the Biological Sciences, G.G Hames, John Wiley & Sons Inc. 2005   

Academic Year: 
2020-2021
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