ANALYTICAL TECHNIQUES

Paper Code: 
BTE 102
Credits: 
3
Contact Hours: 
45
Objective: 

Upon completion of the course the learner will:

CO7: Understand the various analytical techniques used in biological sample analysis

CO8: Recognize the principle and working of Microscopy in sample analysis

CO9: Understand the laws of Spectroscopy and basic principles of Spectroscopic techniques.

CO10: Employ analytical reasoning in biological sample separation through Centrifugation techniques

CO11: Apply scientific knowledge to address research problems using electrophoretic separation techniques.

CO12: Employ scientific reasoning in analyzing and  addressing practical problems in Chromatography

9.00
Unit I: 
Microscopy
  • Introduction to basic principle and applications: Light microscopy (bright field, dark field, phase contrast and fluorescence microscopy), electron microscopy (TEM and SEM)

 

10.00
Unit II: 
Optical methods of Analysis
  • ·Introduction to basic principles and applications: Beer Lambert’s Law   
  • Spectrophotometry (UV-visible, infra red, AAS), Mass spectroscopy

 

8.00
Unit III: 
Chromatography
  • Working principle, instrumentation and application of:  Course Chromatography, Thin Layer Chromatography, Column Chromatography (Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography)

 

10.00
Unit IV: 
Electrophoretic Techniques
  • Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran), Agarose gel electrophoresis, PAGE, SDS-PAGE, isoelectric focusing

 

 

8.00
Unit V: 
Centrifugation
  • Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (preparative and analytical centrifuges) sedimentation analysis & RCF  

 

ESSENTIAL READINGS: 
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2007.
  • Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc., 2009
  • Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2008
  • Principles and Techniques of Biochemistry and  Molecular Biology ,(7th edition), K Wilson and J Walker (editor), Cambridge University Press, 2010

 

REFERENCES: 

SUGGESTED READINGS:

  • Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2014
  • Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005  
  • Bioinstrumentation, J G Webster, John Wiley & Sons Inc. 2004    

 

E-RESOURCES:

 

JOURNALS:

  • Indian Journal of Experimental Biology
  • Journal of Scientific and Industrial Research

 

Academic Year: 
2022-2023
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