ANALYTICAL TECHNIQUES

Paper Code: 
MBL 102
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

  • Learn various techniques used in Microbiology

Learn applications of techniques in the field of biology

9.00
Unit I: 
Microscopy

Introduction to basic principle and applications:Light microscopy (bright field, dark field, phase contrast, interference, fluorescence microscopy), electron microscopy (TEM and SEM), Confocal Microscopy

9.00
Unit II: 
Chromatography

Principle of Paper Chromatography, Thin Layer Chromatography, Column Chromatography (GC, HPLC), Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography

 

8.00
Unit III: 
Centrifugation

Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF          

10.00
Unit IV: 
Electrophoretic Techniques

Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran).

Agarose gel electrophoresis, PAGE, SDS-PAGE. Zone electrophoresis, Isoelectric focussing, Immuno-electrophoresis

9.00
Unit V: 
Spectroscopy

Introduction to basic principles and applications:Spectrophotometry (UV-visible, infra red, AAS), Fluorimetry, NMR, Mass spectroscopy

 

ESSENTIAL READINGS: 
  1. Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  2. Principles and Techniques  of Biochemistry and  Molecular Biology, 6th edition,  K Wilson and J Walker (ed.) Cambridge University Press, 2007
REFERENCES: 
  1. Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004    
  2. Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
  3. Spectroscopy for the Biological Sciences, G.G Hames, John Wiley & Sons Inc. 2005  
Academic Year: 
2014-2015
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