Analytical Techniques

Paper Code: 
MBL 102
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

 

  1. Learn various techniques used in Biotechnology for experimentation.
  2. The students will be able to understand the basic principles and working of different instruments present in a Biotechnology lab and their applications.

 

Course Outcomes (COs):

 

 

Course

Learning              outcome (at course level)

Learning                and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

 

CO 11

Approach      in

C     A     test,

 

 

Explain    the    various

teaching:

Semester end

 

 

separation techniques

Interactive

examinations,

 

 

and                         its

Lectures,

Quiz, Solving

 

 

instrumentation.

Discussion,

problems     in

 

 

CO 12

Tutorials,

tutorials,

 

 

Understand      various

Reading

Assignments,

 

 

fundamentals           of

assignments,

Classroom

 

 

spectroscopy,

presentations

interaction

 

 

qualitative             and

 

 

 

Analytica

quantitative     analysis

Learning

 

BTE

l

and          characterize

activities     for

 

102

Techniqu es

functionalities           of biomolecules by using

the students:

Self-learning

 

 

 

spectroscopic

assignments,

 

 

 

techniques.

Effective

 

 

 

CO 13

questions,

 

 

 

Understand Principle,

Seminar

 

 

 

working, ray diagram

presentation,

 

 

 

and     application     of

Giving tasks.

 

 

 

advance microscopes

 

 

 

 

CO 14

 

 

 

 

Understand    different

 

 

 

 

separation techniques

 

 

 

9.00
Unit I: 
Microscopy
  • Introduction to basic principle and applications: Light microscopy (bright field, dark field, phase contrast and fluorescence microscopy), electron microscopy (TEM and SEM)

 

10.00
Unit II: 
Optical methods of Analysis
  • Introduction to basic principles and applications: Beer Lambert’s Law
  • Spectrophotometry (UV-visible, infra red, AAS), Mass spectroscopy

 

8.00
Unit III: 
Chromatography
  • Working principle, instrumentation and application of: Paper Chromatography, Thin Layer Chromatography, Column Chromatography (Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography)

 

 

10.00
Unit IV: 
Electrophoretic Techniques
  • Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran), Agarose gel electrophoresis, PAGE, SDS-PAGE, isoelectric focusing

 

 

Unit V: 
Centrifugation
  • Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (preparative and analytical centrifuges) sedimentation analysis & RCF

 

 

ESSENTIAL READINGS: 
  • Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  • Principles and Techniques of Biochemistry and Molecular Biology, 6th edition, K Wilson and J Walker (ed.) Cambridge University Press, 2007
  • Bioinstrumentation, J G Webster, John Wiley & Sons Inc. 2004
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
  • Spectroscopy for the Biological Sciences, G.G Hames, John Wiley & Sons Inc. 2005

 

Academic Year: 
2021-2022
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