ANIMAL BIOTECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY

Paper Code: 
24DBTE501A
Credits: 
04
Contact Hours: 
60
Objective: 

This course will enable the students to understand the basics of animal cell science, learn the basic requirement of animal cell culture and its commercial aspect.

10.00
Unit I: 
Introduction to animal cell culture

Structure, organization and biology of animal cell

Cytoskeleton: Self-assembly and dynamic structure of cytoskeletal filaments, actin filament, microtubules, Cell –Cell interaction: cell adhesion, tight and gap junctions, plasmodesmata. General concepts of animal cell culture: Proliferation, differentiation and dedifferentiation, Cell Culture laboratory: basic requirement, set up

9.00
Unit II: 
Growth requirements and kinetics

Media -Development of simple growth media, BSS, Chemical, physical and metabolic functions of different constituents of culture medium, Role of CO2 and serum, Serum and protein free defined media and their applications, Growth kinetics of cultured cells

11.00
Unit III: 
Types of culture and techniques used in ACC

Primary culture and cell line formation, Cell lines: A general concept, finite and infinite cell lines, Organ, organotypic and histotypic cultures, Cell separation, Cell cloning and micromanipulation

15.00
Unit IV: 
Recombinant DNA Technology used in animal cell

Cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC, Enzymes used in gene manipulation- DNA polymerase, Polynucleotide kinase, ligase, terminal deoxynucleotidyl transferase, Reverse transcriptase, Restriction endonucleases, Transfection techniques, Blotting Techniques- Southern, Northern and Western Blotting, Expression of proteins in animal cells: GISH, FISH, microarray

15.00
Unit V: 
Scale-up and application of animal cell culture

Scaling-up of animal cells, Cell culture-based vaccines, Stem cell culture, embryonic stem cells and their applications, Cell hybridization, Hybridomas and their selection, application of cell hybridization, 3 D printing

ESSENTIAL READINGS: 

Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley 2005.

•     Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000

•     Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998

•     Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford, 2000

•     Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004

•     Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003.

REFERENCES: 

Animal Cell Culture by Mohamed Al-Rubeai, Springerlink 2015.

•     Animal Cell Culture: Essential Methods, Edited by Davis, Wiley 2011

 

Academic Year: 
2024-2025
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