Animal Cell Science and Technology

Paper Code: 
BTE 502
Credits: 
03
Contact Hours: 
45
Objective: 

This course will enable the students to -

 

1.     understand the basics of Animal Cell Sciences

2.     learn the basic requirements for Animal Cell Culture

3.     Learn the various types of Animal Cell Culture and techniques used in Animal Cell Culture

study the commercial aspect of Animal Biotechnology

Course Outcomes (COs): 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 502

Animal Cell Science and Technology

 

CO 64 Knowing and understanding the principles of cell culture techniques Study different types of transport mechanisms.

 

CO 65 Knowing required conditions for cell cultures, knowing of media and the equipment required for cell culture.

 

CO 66 Knowing and understanding the cell culture problems and possibilities.

 

CO 67 Study the commercial aspect of Animal Biotechnology

 

CO 68 Learn the application of cell hybridization.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction

 

 
8.00
Unit I: 
General Concept of cell

Structure, organization and biology of animal cell

General concepts of animal cell culture

Proliferation, differentiation and dedifferentiation

Expression of proteins in animal cells

Cell Culture laboratory

 

8.00
Unit II: 
Growth requirements and kinetics

                                               

Media -Development of simple growth media, BSS-Chemical, physical and metabolic functions of different constituents of culture medium-Role of CO2 and serumSerum and protein free defined media and their applications. Growth kinetics of cultured cells

11.00
Unit III: 
Animal cell culture and its types

                                                          

      Primary and established cell line cultures

Cell lines: A general concept

Disaggregation of tissue and primary culture

Cell separation

      Organ and histotypic cultures

10.00
Unit IV: 
Techniques used in Animal Cell Culture

                

Cell cloning and micromanipulation

Transfection techniques

Cell transformation

      Scaling-up of animal cells

8.00
Unit V: 
Application of animal cell culture

        Cell culture based vaccines

Stem cell culture, Embryonic stem cells and their applications

      Cell hybridization, Hybridomas and their selection, application of cell hybridization

ESSENTIAL READINGS: 
  1. Culture of Animal Cells by R. Ian Freshney, 4th  edition, Wiley-Liss, 2000
  2. Animal Cell Culture-Practical Approach by John R.W. Masters, 3rd edition, Oxford Press, 2000
REFERENCES: 
  1. Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
  2. Animal Cell Culture Methods, Jennie P. Mather and David Barnes, Vol 57, Academic Press, 1998
  3. Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
  4. Principles of Gene Manipulation, S.B. Primrose, R.M. Twyman and R.W. Old, 6th edition, Blackwell Science, 2003

 

Academic Year: 
2020-2021
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