ANIMAL CELL SCIENCE AND TECHNOLOGY

Paper Code: 
BTE 421
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

 Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course students will:

CO 83: Understand the basics of animal cell culture

CO 84: Identify growth requirements and kinetics of animal cells.

CO 85: Employ analytical reseasoning in using techniques for in vitro culture of cells

CO 86: Solve problems in Biomedical sciences using Animal Cell Culture related techniques

CO 87: Acquint themselves with cell culture based manipulations for scale up

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

  
 

 

 

15.00
Unit I: 
General Concept of cell proliferation
  • Structure, organization and biology of animal cell, Principles of animal cell culture, need to express in animal cell, Equipments and materials for animal cell culture technique. Biosafety levels and guidelines
  • General concept of differentiation and dedifferentiation

 

15.00
Unit II: 
Growth requirements and kinetics
  • Media Development of simple growth media, Introduction to balanced salt solution, Chemical, physical and metabolic functions of different constituents of culture medium, Role of CO2, Role of serum supplements, Serum and protein free defined media and their applications, Biology and characterization of cultured cells, measuring parameters of growth
15.00
Unit III: 
Animal cell culture and its types
  • Primary and established cell line cultures, Cell lines- Introduction, diversity and selection, nomenclature, routine maintenance and cryopreservation, Disaggregation of tissue and primary culture, Cell separation, Organ and histotypic cultures.

 

15.00
Unit IV: 
Characterization and manipulation of cells
  • Measurement of viability and cytotoxicity, Cell synchronization, Cell cloning and micromanipulation, Cell transformation, Scaling-up of animal cells, Measurement of cell death, Apoptosis.

 

 

15.00
Unit V: 
Applications of animal cell culture
  • Three dimensional culture and tissue engineering, Stem cell culture, embryonic stem cells and their applications, Cell culture based vaccines, Somatic cell fusion, Cell hybridization, Hybridomas and their selection, application of cell hybridization. In vitro testing of drugs and toxicity of environmental pollutants in animal cell culture
ESSENTIAL READINGS: 

Essential Reading:

  • Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley-Liss. 2005.
  • Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000 
  • Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998
  • Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
  • Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
  • Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003.

Suggested Reading:

  • Animal Cell Culture by Mohamed Al-Rubeai, Springerlink 2015.
  • Animal Cell Culture: Essential Methods, Edited By Davis, Wiley 2011

 

 

REFERENCES: 

e resources:

JOURNALS: 

 

 

Academic Year: 
2022-2023
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