ANIMAL CELL SCIENCE AND TECHNOLOGY

Paper Code: 
BTE 502
Credits: 
3
Contact Hours: 
45
Objective: 

Upon completion of the course the learner will:

CO 68: Understand the principles of cell culture techniques Study different types of transport mechanisms.

CO 69: Identify required conditions for cell cultures, knowing of media and the equipment required for cell culture.

CO 70: understand the cell culture problems and possibilities.

CO 71: Apply the knowledge of commercial aspect of Animal Biotechnology

CO 72: Learn the application of cell hybridization.

8.00
Unit I: 
General Concept of cell
  • Structure, organization and biology of animal cell
  • General concepts of animal cell culture
  • Proliferation, differentiation and dedifferentiation
  • Expression of proteins in animal cells
  • Cell Culture laboratory

 

 

8.00
Unit II: 
Growth requirements and kinetics
  • Media -Development of simple growth media, BSS
  • Chemical, physical and metabolic functions of different constituents of culture medium
  • Role of CO2 and serum
  • Serum and protein free defined media and their applications
  • Growth kinetics of cultured cells

 

 

11.00
Unit III: 
Animal cell culture and its types
  • Primary and established cell line cultures
  • Cell lines: A general concept
  • Disaggregation of tissue and primary culture
  • Cell separation
  • Organ and histotypic cultures

 

 

10.00
Unit IV: 
Techniques used in Animal Cell Culture
  • Cell Cloning and micromanipulation
  • Transfection techniques
  • Cell transformation
  • Scaling-up of animal cells

 

 

8.00
Unit V: 
Application of animal cell culture
  • Cell culture based vaccines
  • Stem cell culture, Embryonic stem cells and their applications
  • Cell hybridization, Hybridomas and their selection, application of cell hybridization

 

 

ESSENTIAL READINGS: 
  • Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley-Liss. 2005.
  • Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000 
  • Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998
  • Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
  • Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
  • Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003.

 

 

REFERENCES: 

Suggested Reading:

  • Animal Cell Culture by Mohamed Al-Rubeai, Springerlink 2015.
  • Animal Cell Culture: Essential Methods, Edited By Davis, Wiley  2011

 

e Resources:

JOURNALS:

 

Academic Year: 
2022-2023
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