ANIMAL CELL SCIENCE AND TECHNOLOGY

Paper Code: 
BTE 421
Credits: 
5
Contact Hours: 
75
Objective: 

to enable students to-

  • learn the basics of Animal cell culture.
  • know the various growth requirements and concept of differentiation of animal cells in vitro conditions.
  • become acquainted with the techniques for manipulating the cells lines in laboratory conditions.
  • know the applications of animal cell culture.
15.00
Unit I: 
General Concept of cell proliferation

Structure, organization and biology of animal cell, Principles of animal cell culture, need to express in animal cell, Equipments and materials for animal cell culture technique

General concept of differentiation and dedifferentiation

15.00
Unit II: 
Growth requirements and kinetics:

Media Development of simple growth  media , Introduction to balanced salt solution, Chemical, physical and metabolic functions of different constituents of culture medium, Role of CO2, Role of serum supplements, Serum and protein free defined media and their applications, Biology and characterization of cultured cells, measuring parameters of growth.

15.00
Unit III: 
Animal cell culture and its types

Primary and established cell line cultures, Cell lines- Introduction, diversity and selection, nomenclature, routine maintenance and cryopreservation, Disaggregation of tissue and primary culture, Cell separation, , Organ and histotypic cultures

15.00
Unit IV: 
Characterization and manipulation of cells

Measurement of viability and cytotoxicity, Cell synchronization, Cell cloning and micromanipulation, Cell transformation, Scaling-up of animal cells, Measurement of cell death, Apoptosis.

15.00
Unit V: 
Applications of animal cell culture

Three dimensional culture and tissue engineering, Application of animal cell culture, Stem cell culture, embryonic stem cells and their applications, Cell culture based vaccines, Somatic cell fusion, Cell hybridization, Hybridomas and their selection, application of cell hybridization

ESSENTIAL READINGS: 
  1. Culture of Animal Cells, (4th edition), R. Ian Freshney, Wiley-Liss. 2000
  2. Animal Cell Culture-Practical Approach, John R.W. Masters (editor), Oxford.2000
REFERENCES: 
  1. Methods in Cell Biology, Vol.57, Animal Cell Culture Methods. Jenni P Mather and David Barnes. Academic Press. 1998
  2. Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
  3. Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
  4. Principles of Gene Manipulation, (6th edition) S.B. Primrose, R.M. Twyman and R.W. Old, Blackwell Science, 2003

 

Academic Year: 
2016-2017
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