Animal Cell Science and Technology

Paper Code: 
BTE 502
Credits: 
03
Contact Hours: 
45
Objective: 

This course will enable the students to -

 

  1. understand the basics of Animal Cell Sciences
  2. learn the basic requirements for Animal Cell Culture
  3. Learn the various types of Animal Cell Culture and techniques used in Animal Cell Culture
  4. study the commercial aspect of Animal Biotechnology

 

Course Outcomes (COs):

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

 

 

 

 

 

 

 

 

 

BTE 502

 

 

 

 

 

 

 

 

 

Animal Cell

Science and Technology

CO 64 Knowing and understanding the principles of cell culture techniques Study different types of transport mechanisms.

 

CO 65 Knowing required conditions for cell cultures, knowing of media and the equipment required for cell culture.

Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students: Self-learning assignments, Effective questions, Seminar presentation, Giving

tasks.

C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction

 

 

CO 66 Knowing and understanding the cell culture problems and possibilities.

 

 

 

 

CO 67 Study the commercial aspect of Animal Biotechnology

 

 

 

 

CO 68 Learn the

application of cell hybridization.

 

 

 

8.00
Unit I: 
General Concept of cell
  • Structure, organization and biology of animal cell
  • General concepts of animal cell culture
  • Proliferation, differentiation and dedifferentiation
  • Expression of proteins in animal cells
  • Cell Culture laboratory

 

8.00
Unit II: 
Growth requirements and kinetics
  • Media -Development of simple growth media, BSS
    • Chemical, physical and metabolic functions of different constituents of culture medium
    • -Role of CO2 and serum
  • Serum and protein free defined media and their applications
  • Growth kinetics of cultured cells

 

11.00
Unit III: 
Animal cell culture and its types
  • Primary and established cell line cultures
  • Cell lines: A general concept
  • Disaggregation of tissue and primary culture
  • Cell separation
  • Organ and histotypic cultures

 

10.00
Unit IV: 
Techniques used in Animal Cell Culture
  • Cell Cloning and micromanipulation
  • Transfection techniques
  • Cell transformation
  • Scaling-up of animal cells

 

8.00
Unit V: 
Application of animal cell culture
  • Cell culture based vaccines
  • Stem cell culture, Embryonic stem cells and their applications
  • Cell hybridization, Hybridomas and their selection, application of cell hybridization

 

Academic Year: 
2021-2022
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