Animal Cell Science and Technology

Paper Code: 
BTE 502
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

  • understand the basics of Animal Cell Sciences
  • learn the basic requirements for Animal Cell Culture
  • learn the various types of Animal Cell Culture and techniques used in Animal Cell Culture
  • study the commercial aspect of Animal Biotechnology

 

8.00
Unit I: 
General Concept of cell

Structure, organization and biology of animal cell

General concepts of animal cell culture

Proliferation, differentiation and dedifferentiation

Expression of proteins in animal cells

       Cell Culture laboratory

8.00
Unit II: 
Growth requirements and kinetics

Media -Development of simple growth media, BSS-Chemical, physical and metabolic functions of different constituents of culture medium

-Role of CO2 and serum

Serum and protein free defined media and their applications

       Growth kinetics of cultured cells

11.00
Unit III: 
Animal cell culture and its types

Primary and established cell line cultures

Cell lines: A general concept

Disaggregation of tissue and primary culture

Cell separation

Organ and histotypic cultures

 

10.00
Unit IV: 
Techniques used in Animal Cell Culture

Cell cloning and micromanipulation

Transfection techniques

Cell transformation

Scaling-up of animal cells

 

8.00
Unit V: 
Application of animal cell culture

Cell culture based vaccines

Stem cell culture, Embryonic stem cells and their applications

Cell hybridization, Hybridomas and their selection, application of cell hybridization

 

ESSENTIAL READINGS: 
  1. Culture of Animal Cells by R. Ian Freshney, 4th  edition, Wiley-Liss, 2000
  2. Animal Cell Culture-Practical Approach by John R.W. Masters, 3rd edition, Oxford Press, 2000

 

REFERENCES: 
  1. Basic Cell Culture edited by J.M. Davis 2nd edition, Oxford,2000
  2. Animal Cell Culture Methods, Jennie P. Mather and David Barnes, Vol 57, Academic Press, 1998
  3. Protein Expression: A Practical Approach, S.J. Higgins and B.D. Hames, Oxford University Press, 2004
  4. Principles of Gene Manipulation, S.B. Primrose, R.M. Twyman and R.W. Old, 6th edition, Blackwell Science, 2003

 

Academic Year: 
2019-2020
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