APPLIED ENVIRONMENTAL MICROBIOLOGY

Paper Code:

MBL 325 A

Credits:

Contact Hours:

Objective:

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 96 A: Acquaint with role of microbes in solid and liquid waste management

CO 97 A: Assess role of microbes in biodegradation and bioremediation of environmental pollutants

CO 98 A: Assess the role microorganisms in biodeterioration and bioleaching of metals.

CO 99 A: Assess the role of biofertilizers and methods of inoculation

CO 100 A: Assess mycorrhizal relationship, bioplastics and biopesticides..

Interactive Lectures, Discussion, Tutorials, Reading assignments, Self-learning assignments, Effective questions, Giving tasks

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

18.00

Unit I:

Waste management

Treatment of industrial effluents and municipal waste through microorganisms.
Aerobic Processes: Oxidation pools, Rotating Biological Discs, Rotating Drums, Anaerobic Processes: Anaerobic digestion, Anaerobic filter, upflow anaerobic sludge blanket reactors. Indicator microorganisms.
Solid wastes: Sources and management (composting, vermiculture and Methane production

15.00

Unit II:

Biodegradation

· Biodegradation of natural compounds (cellulose, hemicelluloses, lignin, chitin,)

· Biodegradation of xenobiotics in environment – Organisms involved in degradation of chlorinated hydrocarbons, substituted simple aromatic compounds, polyaromatic hydrocarbons, pesticides, synthetic polymers, detergents and hydrocarbons

Bioremediation- exsitu and in situ. bioaccumulation, biomagnifications

15.00

Unit III:

Biodeterioration and Bioleaching

Definition, biodetorioration of paper, wood, paint, textiles, leather, metals (corrosion). Control of biodetorioration.
Microorganisms and metal pollutants- metal bioavalibility in environment, mechanism of microbial metal resistance and detoxification, metal- microbe interaction, Bioleaching of metals, Microbial enhanced oil recovery.

15.00

Unit IV:

Biofertilizers

Definition and types of biofertilizers, Mass cultivation and methods of inoculation of microbial inoculants – (Rhizobium, Azotobacter, &Asospirillium.) Cyanobacteria – Azolla – Anabaena association and its role in rice cultivation
Quality control and ISI specifications for Rhizobium cultures.

12.00

Unit V:

Mycorrhizal Relationship, Biopesticides and Bioplastics

AMF mycorrhizal association, Types of mycorrhizal association, Isolation, stock plants and inoculum production of AMF.
Physiology and function of mycorrhizae- Nutrient Uptake and other effects.
Biopesticides (with special emphasis on B. thruingenesis.)
Bioplastics

ESSENTIAL READINGS:

Plant, Gene and crop Biotechnology, M.J. Chrispeel and D.E. Sadava ASPB 2003.
Textbook of Environmental Biotechnology, P.K Mohapatra, I K International Publications, Mumbai 2006.
Environmental Chemistry, A.K.De, Willey Eastern Ltd., New Delhi.
Microbial Ecology- Fundamentals and applications. Ronald. M. Atlas, Richard Bartha. Pearson Education. 2005.
Environmental Biotechnology – Basic concepts and applications, InduShekhar Thakur, I K International publications. 2006

REFERENCES:

SUGGESTED READINGS:

· Wastewater Engineering- Treatment, Disposal and Resuse, Metcalf and Eddy, Inc., Tata McGraw Hill, Delhi.1999

· Comprehensive Biotechnology, vol.4, M. Moo- Young (Ed-in-chief), Pergamon Press, Oxford. 2004

· Bioextraction and Biodeterioration of Metals (Biology of World Resources. Christine C. Gaylarde (Editor), Hector A. Videla (Editor), Dennis Allsopp (Foreword). Cambridge University Press. 2009

· Handbook of Material Biodegradation, Biodeterioration, and Biostablization. Michalina Falkiewicz-Dulik, Katarzyna Janda Dr., George Wypych. ChemTec Publishing. 2010

· Environmental Biotechnology. Theory and Applications. Gareth M. EvansJudith C. Furlong. Wiley. 2003

e RESOURCES:

· Environmental Biotechnology - Course (nptel.ac.in)

· Environmental biotechnology (slideshare.net)

· Untitled Document (ernet.in)

https://youtu.be/Hwi661vA8EA

JOURNALS: