BACTERIOLOGY

Paper Code: 
MBL 121
Credits: 
05
Contact Hours: 
75
Objective: 

This course will enable the students to –

  1. To learn about structure of bacterial cell
  2. Have an idea about characters of different Eubacteria and Archaebacteria
  3. To understand growth and preservation of microbes.
  4. understand classification and taxonomy of bacteria

 

Course Outcomes (COs): 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

MBL 121

 

Bacteriology

 

Upon completion of the course students will be able to

CO 1: Inculcate knowledge about structure of bacterial cell.

CO 2: Assess major characteristics of Eubacteria and Archaebacteria.

CO 3: Gain insight on microbial growth  and preservation.                                            CO 4: Evaluate and interpret different classification systems according to Bergey’s manual.

CO 5: Understand  chemotaxonomy and genetic methods of taxonomy .

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

 

 
15.00
Unit I: 
Ultra structure of bacteria

Cell wall structure and synthesis, cell membrane,. Flagella and motility, chemotaxis Pili, Cell inclusions like Glycogen granules, Volutin granules, Carboxysomes etc, Slime sheet and capsule.

Endospre structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospres

15.00
Unit II: 
Eubacteria and Archaebacteria

General characters and structure of Spirochetes, cyanobacteria, purple and green bacteria, rickettsia, Chlamydia and mycoplasma                                                               

Gram positive bacteria- endospore forming bacteria, actinomycetes, mycobacteria. Archaebacteria-methanotrophs, halophiles and sulphur bacteria.

15.00
Unit III: 
Microbial Growth

The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH , activity of water and gaseous environment, maintenance and preservation of microorganisms

 

12.00
Unit IV: 
Classification

Classification of microorganisms-introduction,Haekels three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains

Classification and salient features of bacteria according to Bergey’s manual of systematic bacteriology(a brief outline)

Morphlogical types of bacteria

Nutritional classification of bacteria

18.00
Unit V: 
Taxonomy

Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence of

proteins, protein profiles, cytochrome composition , ribosomal RNA, etc)

 Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools for

Bacteria, DNA base composition and hybridisation,  nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers)

Culture collections

ESSENTIAL READINGS: 
  1. Basic and Practical Microbiology, Ronald M Atlas, Mac Millan Company New York, 1989
  2. Biology of Microorganisms, T D Brock, M T Madigan, (10th edition), Pearson education, Inc., 2003
  3. Microbiology , Prescott, Harley, Klein, (6th edition), Mc Graw Hill Companies, 2005
  4. Microbiology Fundamentals and Applications , R M Atlas, (2nd edition), Maxwell Macmillan International edition, 1989
  5. Microbiology-a Laboratory Manual, J G Cappuccino and N Sherman, (6th edition),Addison Wesley, Pearson education, Inc., 2006
  6. Microbial diversity: current perspectives and potential applications, Satynarayana T. & Johri B.N. I.K. International Pvt. Ltd. 2005
  7. Microbial Physiology, (4th edition ), by A.G. Moat ,J.W.Foster and M.P. Spector. Wiley Liss publications 2002.
  8. Benson’s Microbiological application: Laboratory Mannual in general Mirobiology (9th edition), Brown A E, Mc Graw Hill, 2005                            
REFERENCES: 
  1. Foundation in Microbiology, K P Talaro and A Talaro, (4th edition),  Mc Graw Hill, 2002
  2. General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  3. Laboratory Fundamentals of Microbiology, I E Alcamo, Jones and Barlett publishers, 2001
  4. Microbial Diversity, D Colwd, Academic press, 1999.
  5. Microbiology , M. J Pelczar, E C S Chan, N R Kreig, (5th edition), Tata Mc Graw Publication, 2006
  6. Prokaryotic Development, Y V Burn. & L J Shimkets, ASM Press, 2000
Academic Year: 
2020-2021
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