BACTERIOLOGY

Paper Code: 
MBL 121
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

      

  Course Outcomes

Learning and teaching strategies

Assessment Strategies

  
 

On completion of this course, the learner will:

CO 1: Assess the structure of bacterial cell

CO 2: Summarize the major characteristics of Eubacteria and Archaebacteria

CO 3: Interpret the microbial growth and preservation

CO 4:  Evaluate and examine different classification systems according to Bergey’s manual

CO 5: Examine chemotaxonomy and genetic methods of taxonomy

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  

 

15.00
Unit I: 
Ultra structure of bacteria
  • Cell wall structure and synthesis, cell membrane, Flagella and motility, chemotaxis Pili, Cell inclusions like Glycogen granules, Volutin granules, Carboxysomesetc, Slime sheet and capsule.
  • Endospre structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospres
15.00
Unit II: 
Eubacteria and Archaebacteria
  • General characters and structure of Spirochetes, cyanobacteria, purple and green bacteria, rickettsia, Chlamydia and mycoplasma                                              
  • Gram positive bacteria- endospore forming bacteria, actinomycetes, mycobacteria. Archaebacteria-methanotrophs, halophiles and sulphur bacteria.

 

 

 

15.00
Unit III: 
Microbial Growth
  • The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH, activity of water and gaseous environment, maintenance and preservation of microorganisms
12.00
Unit IV: 
Classification
  • Classification of microorganisms-introduction, Haekels three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains
  • Classification and salient features of bacteria according to Bergey’s manual of systematic bacteriology (a brief outline)
  • Morphlogical types of bacteria
  • Nutritional classification of bacteria
18.00
Unit V: 
Taxonomy
  • Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence ofproteins, protein profiles, cytochrome composition, ribosomal RNA, etc)
  • Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools forBacteria, DNA base composition and hybridisation, nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers)
  • Culture collections
ESSENTIAL READINGS: 
  • Microbiology ,5th edition, M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2019
  • Microbiology-a Laboratory Manual, 10th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2014
  • Microbiology-an introduction, 13th edition, G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020
  • Prescott’s Microbiology, 11th edition, J Willey, K Sandman, D Wood, Mc Graw Hill Companies, 2019
  • General microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
  • Microbial diversity: current perspectives and potential applications, Satynarayana T. &Johri B.N. I.K. International Pvt. Ltd. 2005
  • Microbiology: principles and explorations, 9th edition, J G Black, L J Black, John Wiley and Sons, Inc, 2015
REFERENCES: 

SUGGESTED READINGS:

  • Advances in Microbial Physiology, 1st edition, R K Poole, Academic Press, 2006
  • Brock Biology of Microorganisms, 14th edition, M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • General Microbiology, 5th edition, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Fundamentals of microbiology, 11th edition, J C. Pommerville, Jones & Barlett Publishers, 2017
  • Foundation in Microbiology, 11th edition, K P Talaro and A Talaro, Mc Graw Hill, 2021

 

e RESOURCES:

 

JOURNALS:

  • Frontiers in Microbiology
  • Journal of & Letters in Applied Microbiology
  • BMC Microbiology
  • FEMS Microbiology Letters
  • PLOS ONE

 

Academic Year: 
2022-2023
  • Printer-friendly version
  • PDF version