BACTERIOLOGY

Paper Code: 
24MBL124
Credits: 
2
Contact Hours: 
30
Objective: 

Course Objectives: This course will enable the students to assess the morphology, classification, taxonomy, characteristics of Eubacteria and Archaebacteria along with microbial growth analysis.

 

Course Outcomes (COs):

Course

Learning outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course

title

24MBL 124

Bacteriology

(Theory)

CO19: Assess the ultrastructure of bacterial cell and endospore structure and formation

CO20: Explain the general characters and structure of Spirochetes, Rickettsia, Chlamydia, Mycoplasma, some Gram

positive bacteria like Actinomycetes, Mycobacteria and Archaebacteria

CO21: Interpret the microbial growth and preservation

CO22:  Evaluate different classification systems according to Bergey’s manual

CO23: Discuss chemotaxonomy and genetic methods of taxonomy

CO24: Contribute effectively in

course-specific interaction

Approach in teaching:  Interactive lectures,

Demonstration.

 

 

Learning activities for the students:

Group discussions and workshops, Seminars,

Tutorials,

Assignments

Reading Journals

 

 

 Class test, Semester end

  examinations,                     Quiz        

Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

6.00
Unit I: 
Ultra structure of bacteria

Cell wall structure and synthesis, cell membrane, Flagella and motility, chemotaxis Pili. Endospore structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospores.

 

6.00
Unit II: 
Eubacteria and Archaebacteria

General characters and structure of Spirochetes, Rickettsia, Chlamydia and Mycoplasma. Gram positive bacteria- Actinomycetes, Mycobacteria. Archaebacteria-methanotrophs, Halophiles and Sulphur bacteria.

 

6.00
Unit III: 
Microbial Growth

The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH, activity of water and gaseous environment, maintenance and preservation of microorganisms.

 

 

6.00
Unit IV: 
Classification

Classification of microorganisms-introduction, Haeckel’s three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains, Morphological types of bacteria, Nutritional classification of bacteria

 

 

6.00
Unit V: 
Taxonomy

Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence of proteins, protein profiles, cytochrome composition, ribosomal RNA, etc.)

Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools for Bacteria, DNA base composition and hybridization, nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers)

Culture collections.

 

ESSENTIAL READINGS: 

ESSENTIAL READINGS:

  • Microbiology ,5th edition, M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2019
  • Microbiology-a Laboratory Manual, 10th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2014
  • Microbiology-an introduction, 13th edition, G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020
  • Prescott’s Microbiology, 11th edition, J Willey, K Sandman, D Wood, Mc Graw Hill Companies, 2019
  • General microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
  • Microbial diversity: current perspectives and potential applications, Satynarayana T. &Johri B.N. I.K. International Pvt. Ltd. 2005
  • Microbiology: principles and explorations, 9th edition, J G Black, L J Black, John Wiley and Sons, Inc, 2015

SUGGESTED READINGS:

  • Advances in Microbial Physiology, 1st edition, R K Poole, Academic Press, 2006
  • Brock Biology of Microorganisms, 14th edition, M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • General Microbiology, 5th edition, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Fundamentals of microbiology, 11th edition, J C. Pommerville, Jones & Barlett Publishers, 2017
  • Foundation in Microbiology, 11th edition, K P Talaro and A Talaro, Mc Graw Hill, 2021

   e RESOURCES:

   JOURNALS:

  • Frontiers in Microbiology
  • Journal of & Letters in Applied Microbiology
  • BMC Microbiology
  • FEMS Microbiology Letters
  • PLOS ONE

 

 

Academic Year: 
2024-2025
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