BACTERIOLOGY

Paper Code: 
MBL 121
Credits: 
05
Contact Hours: 
75
Objective: 

to enable students to-

  • To learn about structure of bacterial cell
  • Have an idea about characters of different Eubacteria and Archaebacteria
  • To understand growth and preservation of microbes.
  • understand classification and taxonomy of bacteria
15.00
Unit I: 
Ultra structure of bacteria

Cell wall structure and synthesis, cell membrane,. Flagella and motility, chemotaxis Pili, Cell inclusions like Glycogen granules, Volutin granules, Carboxysomes etc, Slime sheet and capsule.

Endospre structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospres

15.00
Unit II: 
Eubacteria and Archaebacteria

General characters and structure of  Spirochetes, cyanobacteria, purple and green bacteria, rickettsia, Chlamydia and mycoplasma                                                               

Gram positive bacteria- endospore forming bacteria, actinomycetes, mycobacteria.

Archaebacteria-methanotrophs, halophiles and sulphur bacteria.

15.00
Unit III: 
Microbial Growth

The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH , activity of water and gaseous environment, maintenance and preservation of microorganisms

12.00
Unit IV: 
Classification

Classification of microorganisms-introduction,Haekels three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains

Classification and salient features of bacteria according to Bergey’s manual of systematic bacteriology(a brief outline)

Morphlogical types of bacteria

Nutritional classification of bacteria

18.00
Unit V: 
Taxonomy

Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence of

proteins, protein profiles, cytochrome composition , ribosomal RNA, etc)

 Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools for

Bacteria, DNA base composition and hybridisation,  nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers)

Culture collections

ESSENTIAL READINGS: 

  1. Basic and Practical Microbiology, Ronald M Atlas, Mac Millan Company New York, 1989
  2. Biology of Microorganisms, T D Brock, M T Madigan, (10th edition), Pearson education, Inc., 2003
  3. Microbiology , Prescott, Harley, Klein, (6th edition), Mc Graw Hill Companies, 2005
  4. Microbiology Fundamentals and Applications , R M Atlas, (2nd edition), Maxwell Macmillan International edition, 1989
  5. Microbiology-a Laboratory Manual, J G Cappuccino and N Sherman, (6th edition),Addison Wesley, Pearson education, Inc., 2006
  6. Microbial diversity: current perspectives and potential applications, Satynarayana T. & Johri B.N. I.K. International Pvt. Ltd. 2005
  7. Microbial Physiology, (4th edition ), by A.G. Moat ,J.W.Foster and M.P. Spector. Wiley Liss publications 2002.
  8. Benson’s Microbiological application: Laboratory Mannual in general Mirobiology (9th edition), Brown A E, Mc Graw Hill, 2005                            
REFERENCES: 
  1. Foundation in Microbiology, K P Talaro and A Talaro, (4th edition),  Mc Graw Hill, 2002
  2. General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  3. Laboratory Fundamentals of Microbiology, I E Alcamo, Jones and Barlett publishers, 2001
  4. Microbial Diversity, D Colwd, Academic press, 1999.
  5. Microbiology , M. J Pelczar, E C S Chan, N R Kreig, (5th edition), Tata Mc Graw Publication, 2006
  6. Prokaryotic Development, Y V Burn. & L J Shimkets, ASM Press, 2000
Academic Year: