BACTERIOLOGY

Paper Code: 
MBL 202
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students:

  • To have an idea about different aspects of applied microbiology
  • To study about bacterial growth and factors affecting it.
  • To learn staining techniques and preservation of microbes
  •  To learn aseptic techniques
  • To learn the different types of microbiological media
7.00
Unit I: 
Scope of Microbiology

Microbiology as a field of biology, a brief idea of some applied areas of microbiology: medical microbiology, aquatic, aero, food, agriculture, industrial and geochemical microbiology

10.00
Unit II: 
Growth of bacteria

Definition of growth, rate of growth, generation time, growth curve, measurement of population growth, batch culture, continuous culture and synchronous growth.

Factors affecting growth. - Temperature, pH, oxygen, water availability.

10.00
Unit III: 
Control of Microorganisms

Physical agents- Moist heat, dry heat, filtration, radiation.

Chemicals as antimicrobial agents major groups of disinfectants, antiseptics and chemical sterilants.

8.00
Unit IV: 
Cultivation of microorganisms

Microbiological media, Physical states and chemical content of media, general purpose and special purpose media. Nutritional classification of Microorganisms.

Isolation techniques (Streak plate, pour plate and spread plate method).

10.00
Unit V: 
Identification and preservation of microorganisms

Preparation of microorganisms for light microscopy- wet mount and hanging drop technique. Staining techniques- simple staining, differential staining (Gram’s staining, acid fast staining, endospore staining). Special staining (capsule staining and flagella staining).

Preservation of microorganisms by sub culturing, overlaying cultures with mineral oil, lyophilization, use of liquid nitrogen.

ESSENTIAL READINGS: 
  1. General Microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
  2. Microbiology ,5th edition M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2006
  3. Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006
  4. Microbiology-an introduction, 9th edition G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2007
REFERENCES: 

  1. Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
  2. General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  3. Laboratory fundamentals of microbiology, I E Alcamo, Jones and Barlett Publishers, 2001
  4. Microbiology , 6th edition, Prescott, Harley, Klein, Mc Graw Hill Companies, 2005
  5. Microbiology Fundamentals and Applications ,2nd edition, R M Atlas, Maxwell Macmillan International Edition, 1989
Academic Year: 
2014-2015
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