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Bacteriology
To enable students to- • To learn about structure of bacterial cell • Have an idea about characters of different Eubacteria and Archaebacteria • To understand growth and preservation of microbes. • understand classification and taxonomy of bacteria
Cell wall structure and synthesis, cell membrane,. Flagella and motility, chemotaxis Pili, Cell inclusions like Glycogen granules, Volutin granules, Carboxysomes etc, Slime sheet and capsule. Endospre structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospres
General characters and structure of Spirochetes, cyanobacteria, purple and green bacteria, rickettsia, Chlamydia and mycoplasma Gram positive bacteria- endospore forming bacteria, actinomycetes, mycobacteria. Archaebacteria-methanotrophs, halophiles and sulphur bacteria
The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH , activity of water and gaseous environment, maintenance and preservation of microorganisms
Classification of microorganisms-introduction,Haekels three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains Classification and salient features of bacteria according to Bergey’s manual of systematic bacteriology(a brief outline) Morphlogical types of bacteria Nutritional classification of bacteria
Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence of proteins, protein profiles, cytochrome composition , ribosomal RNA, etc) Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools for Bacteria, DNA base composition and hybridisation, nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers) Culture collections
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