BASIC MICROBIOLOGICAL TECHNIQUES (Skill Enhancement Course)

Paper Code: 
SMBL 501
Credits: 
02
Contact Hours: 
30
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 133: Assess the internal structure of bacteria with various staining techniques

CO 134: Identify various aseptic techniques to control microbial growth

CO 135: Assess various culture techniques of microbes.

CO 136: Design and formulate different types of medium for microbial growth

CO 137: Screen assays for detection of enzymes and antibiotics

Interactive Lectures,

Discussion,

Tutorials,

Demonstrations,

Assignments

Reading Journals

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

7.00
Unit I: 
Internal Structure of Bacteria and specimen preparations

Bacterial shapes and arrangement, cell membrane, cell wall, endospores, Demonstrations for staining techniques Nature of dyes. Physical and chemical theories of staining- Staining techniques; principle, procedure and applications: i) Simple staining- negative staining, ii) Differential staining-Grams and acid fast staining, iii) Structural staining- cell wall, endospore, flagella and capsular staining.

 

5.00
Unit II: 
Control of microbial growth

Physical methods of microbial control- heat, filtration and radiations, Chemical methods of microbial control- principles of effective disinfection, types of disinfectants, Demonstrations for aseptic techniques

 

8.00
Unit III: 
Microbial growth

Physical and chemical requirements for growth, culture media- chemically defined media, selective and differential media, study of bacterial growth curve, Demonstrations for media preparation and growth curve

 

5.00
Unit IV: 
Pure culture techniques

Pure culture isolation methods- streak plate, pour plate and spread plate methods, serial dilution and standard plate count, Demonstrations for Pure culture isolation methods

 

5.00
Unit V: 
Bioactive assays

Screening assays for detection of enzymes-amylase, protease, lipase, gelatinase, Assay of antibiotics

 

ESSENTIAL READINGS: 
  • Microbiology,5th edition M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2019
  • Microbiology-a Laboratory Manual, 10th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2014
  • Microbiology-an introduction, 13th edition G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020
  • Prescott’s Microbiology, 11th edition, J Willey, K Sandman, D Wood,  Mc Graw Hill Companies, 2019

 

REFERENCES: 
  • D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • General Microbiology, 5th edition R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Fundamentals of microbiology, 11th edition J C. Pommerville, Jones &Barlett Publishers, 2017
  • Microbiology Fundamentals and Applications ,2nd edition, R M Atlas, Maxwell Macmillan International Edition, 1989

 

e RESOURCES:

 

JOURNALS:

  • Frontiers in Microbiology
  • Journal of & Letters in Applied Microbiology
  • FEMS Microbiology Letters
  • PLOS ONE
  • Nature

 

 

 

Academic Year: 
2023-2024
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