Biochemistry and Instrumentation

Paper Code: 
24CBTE101
Credits: 
04
Contact Hours: 
60
Objective: 

This course will enable the students to build upon undergraduate level knowledge of biochemical principles with specific emphasis on different metabolic pathways. The course shall make the students aware of the new technologies that current experimental researchers are employing to prove complex biology questions in life-sciences

 

12.00
Unit I: 
Carbohydrates & Lipids

Structure of monosaccharide (trioses, pentoses & hexoses), disaccharides (sucrose, maltose,              lactose), polysaccharides (starch, glycogen, cellulose, peptidoglycan). Fundamental Properties: Stereoisomerism and mutarotation. Structure and nomenclature of fatty acids. Classification of lipids (triacylglycerols, phospholipids, sphingolipids, sterols).Membrane lipids. Biological functions of lipids

12.00
Unit II: 
Nucleic Acids & Proteins

Biochemical structure & functions of Nucleic Acids. The central Dogma and its exceptions. Structure of essential and non-essential amino acids. Peptide bond, structural hierarchy of proteins. Protein classification on the basis of structure and function. Biological functions of proteins

 

 

12.00
Unit III: 
Buffers & Enzymology

Buffers: Concept of pH, pK, acids, bases. Classification and nomenclature of enzymes. Mechanism of enzyme action, Michaelis- Menten kinetics. Coenzymes, Enzyme inhibition, Regulation of enzyme activity

12.00
Unit IV: 
Instrumentation I: Spectroscopy, Chromatography

Introduction to basic principles and applications of Beer Lambert’s Law, UV-Vis Spectroscopy. Chromatography: Working principle, instrumentation and application of:  Paper Chromatography, Thin Layer Chromatography and Column Chromatography

 

12.00
Unit V: 
Instrumentation II: Electrophoresis, Centrifugation & Microscopy

Principle of Electrophoresis, Agarose Gel Electrophoresis, PAGE, SDS-PAGE, 2-D electrophoresis. Principle of centrifugation. Types of rotors. Differential and density gradient, ultracentrifugation (Preparative and analytical centrifuges. Sedimentation analysis & RCF. Microscopy: construction of a microscope, light microscopy (bright field, dark field, phase-contrast, interference, confocal, polarization microscopy).

ESSENTIAL READINGS: 
  1. Biochemistry, D.Voet and J.G.Voet, John Wiley & Sons, 2nd Edition, 1995.
  2. Lehninger Principles of Biochemistry, Nelson and Cox, 3rd Edition, 1999.
  3. Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, 6th Edition, 2007.
  4. Biochemistry and Molecular Biology by W.H. Elliot and Daphne C. Elliot, Oxford University Press, 3rd Edition, 2005.
  5. Biochemistry, J. David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.

 

REFERENCES: 
  1. Physical Biochemistry, D Friefelder, W.H. Freeman & Company, 17th Reprint, 1999.
  2. Understanding Chemistry, CNR Rao, Universities Press, Hyderabad 1999.
  3. A Biologist’s Guide to Principles and Techniques of Practical Biochemistry, K.Wilson & KH Goulding, ELBS Edition 1986.
  4. Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications,7th Reprint, 2001.
  5. Encyclopedia of Molecular Biology, J.Kendrew, Blackwell Scientific Publications, Oxford.
  6. Instant Notes- Biochemistry by B.D. Hames and N.M. Hooper, Viva Books Pvt, Ltd., 1st Indian Edition, 2001.
Academic Year: 
2024-2025
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