Bioprocess engineering and technology

Paper Code: 
BTE 423
Credits: 
5
Contact Hours: 
75
Objective: 

 

to enable students to-

  • get an overview of design of Bioreactor types
  • learn various techniques of isolation and screening of industrially important micro organisms.
  • understand the rationale of medium formulation for bioprocesses.

understand downstream processing and whole cell immobilization

15.00
Unit I: 
Introduction of bioprocess engineering

Bioreactors:  Conventional and Specialized bioreactors (pulsed, fluidized, photo bioreactors), Types of fermentation processes batch, fed batch and continuous cultures. Energy requirement, yield, P/O quotients, analysis of mixed microbial populations.

15.00
Unit II: 
Isolation and screening strategies of industrially important cultures

Isolation of cultures, screening for activities, inoculum preparation Scale up and pilot plants. Microbial kinetics of growth and death, Culture preservation, maintenance, Strain improvement.

15.00
Unit III: 
Medium formulation and control of bioprocess parameters

Media for industrial fermentation, Air and media sterilization, Process control ( various parameters;: pH, temperature, oxygen, foam)

15.00
Unit IV: 
Downstream processing

Introduction, Removal of microbial cells and solid matter, foam separation, cell disruption, precipitation, filtration, centrifugation, liquid-liquid extraction, chromatography, membrane process, Drying and crystallization.,

15.00
Unit V: 
Enzyme and Whole cell immobilization

Techniques of immobilization. Application of immobilization in Biotechnology. Biosafety

ESSENTIAL READINGS: 
  1. Bioprocess Engineering  (2nd edition) by Michael L.Schuler and F.Kargi .Prentice Hall of  India.2002.
  2.  Biochemcal Engineering Fundamentals, (2nd edition).  J.E. Bailey and D.F. Ollis Mcgraw Hill International Editions 1986.
  3. Process Biotehnology Fundamentals (2nd edition0 by S.N. Mukhopadhyaya. Vivi Books Pvt. Ltd.2004
  4. Principles of Fermentation Technology by  P.F. Stanbury,A. Whittaker & Hall    Pergaman. McNeul & Harvey Publications. 1990.
REFERENCES: 

1. Fermentation Microbiology and Biotechnology Edited by E.M.T. El- Mansi and C.F.A. Bryce.Taylor and Francis Group 2005.

2. Manual of Industrial Microbiology  and Biotechnology(2nd edition). Edited  by A.L. Demain and J. E Davies, Panima Publications 2004

3. Comprehensive Biotechnology Vol I-IV   Editors  Moo Young et al . 1989.

Academic Year: 
2018-2019
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