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Bioprocess Engineering and Technology | Biotechnology

Bioprocess Engineering and Technology

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Paper Code: 
25MBL328
Credits: 
04
Contact Hours: 
120
Objective: 

This course will enable the students to build upon postgraduate level knowledge of technology involved in bioprocess engineering. The course shall impart the students with hands-on training in basic Microbiology and fermentation experimental set up and data analysis along with understanding of unit operations. 

Course Outcomes: 

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25MBL328

Laboratory V: Bioprocess Engineering and Technology (Practical)

CO152: Take part in isolation of

microorganism from soil, scaling up

of cultures and list the related

instrumentation

CO153:Mark the experimental set

up of bioreactor and assess the

parameters for growth kinetics,

substrate and product inhibitors and residual substrates

CO154: Analyze types of

fermentation cultures along with

unit operations and metabolic flux

analysis and analytical techniques

CO155: Identify and illustrate the

various bioanalytical tools.

CO156: Discuss and defend the concepts of Bioprocess Engineering and fermentation technology and illustrate the exercises performed with appropriate methods and outcomes and maintain proper documentation of the same.

CO157: Contribute effectively in course-specific interaction

Approach in teaching:  Interactive Lectures,                  

Demonstrations

 

Learning activities for the students:  Hands-on practical, Discussion, Reading of journals and protocols

 

Class test, Semester end examinations Hands-on practical assessment, file making, viva voce

Experiments based on:

Bioprocess Engineering and Fermentation Technology

1. Basic techniques

 a) Scale up from frozen vial to agar plate to shake flask culture.

 b) Instrumentation: Microplate reader, spectrophotometer, microscopy.

 c) Isolation of microorganisms from soil samples.

2. Experimental set-up

 a) Assembly of bioreactor and sterilization.

 b) Growth kinetics.

 c) Substrate and product inhibitions.

 d) Measurement of residual substrates.

3. Data Analysis

 a) Introduction to Metabolic Flux Analysis (MFA).

4. Fermentation

 a) Batch.

 b) Fed-batch.

 c) Continuous.

5. Unit operations

 a) Microfiltrations: Separation of cells from broth.

 b) Bioseparations: Various chromatographic techniques and extractions.

6. Bioanalytics

 a) Analytical techniques like HPLC, FPLC, GC, GC-MS etc. for measurement of amounts of products/substrates

 

Academic Year: